CORE--Cell Biology / Morphology

细胞生物学/形态核心(核心D)提供一整套生物成像服务 协助COPD SCCOR调查人员需要照片和缩微文件 (可见、荧光和电子)基础和临床研究数据。除了成像之外 服务，核心提供其在样品处理和检测系统方面的经验 (免疫组织化学、免疫荧光、原位杂交、扫描和传播 电子显微镜)，以分析实验样本中的基因和蛋白质表达。 核心的工作人员将与项目调查人员合作开发新的应用程序和 对COPD Scorr中产生的数据进行分析。核心D贡献的具体实例 将包括对大型群体中细胞类型量化的项目的援助 和小气道上皮和支气管肺泡灌洗液样本作为起始材料 对于基因表达的分析，对呼吸道进行免疫组织化学研究 上皮细胞和肺泡巨噬细胞群体评估所选基因在 形态水平，分析支气管活检以量化细胞类型所占比例 与刷牙样本比较，免疫组织化学分析筛选出 基因，细胞周期分析，光镜下纤毛细胞的形态分析 用透射电子显微镜测定纤毛节拍的纤毛水平和纤毛 在肺再生和肺再生模型中肺气肿程度的频率和量化 量化循环干细胞所贡献的实质细胞比例。

The Cell Biology/Morphology Core (Core D) provides a full array of biologic imaging services to aid COPD SCCOR investigators requiring photographic and micrographic documentation (visible, fluorescence, and electron) of basic and clinical research data. In addition to imaging services, the Core makes available its experience with sample handling and detection systems (immunohistochemistry, immunofluorescence, in situ hybridization, scanning and transmission electron microscopy) required to analyze gene and protein expression in experimental samples. The staff of the Core will work with project investigators to develop new applications and analyses for data generated in the COPD SCORR. Specific examples of Core D contributions will include assistance to the Projects in quantification of cell types in the populations of large and small airway epithelium and bronchoalveolar lavage samples that are the starting material for the analyses of gene expression, carrying out immunohistochemical studies on the airway epithelial and alveolar macrophage populations to to assess the expression of selected genes at the morphologic level, analysis of bronchial biopsies to quantify the proportion of cell types to compare to the brushed samples, immunohistochemical analysis of the expression of selected genes, cell cycle analysis, analysis of the morphology of ciliated cells at the light microscopy level and of cilia perse using transmission electron microscopy, determination of cilia beat frequency, and quantitation of the extent of emphysema in the models of lung regeneration and to quantify the proportion of parenchymal cells contributed by circulating stem cells.