CORE--Cell Biology / Morphology

核心--细胞生物学/形态学

• 批准号: 7394196
• 负责人: PHILIP L LEOPOLD
• 金额: $ 20.15万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-12 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7394196
• 关键词: Alveolar Macrophages; Biopsy; Bronchoalveolar Lavage; Cells; Cellular biology; Chronic Obstructive Airway Disease; Cilia; Clinical Research; Data; Data Analyses; Detection; Documentation; Electrons; Epithelial; Fluorescence; Frequencies; Gene Expression; Gene Proteins; Genes; Image; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Lung; Modeling; Morphology; Natural regeneration; Population; Pulmonary Emphysema; Research Personnel; Sampling; Scanning Transmission Electron Microscopy Procedures; Services; Stem cells; System; Transmission Electron Microscopy; Work; airway epithelium; cdc Genes; cell type; experience; light microscopy; protein expression

The Cell Biology/Morphology Core (Core D) provides a full array of biologic imaging services to aid COPD SCCOR investigators requiring photographic and micrographic documentation (visible, fluorescence, and electron) of basic and clinical research data. In addition to imaging services, the Core makes available its experience with sample handling and detection systems (immunohistochemistry, immunofluorescence, in situ hybridization, scanning and transmission electron microscopy) required to analyze gene and protein expression in experimental samples. The staff of the Core will work with project investigators to develop new applications and analyses for data generated in the COPD SCORR. Specific examples of Core D contributions will include assistance to the Projects in quantification of cell types in the populations of large and small airway epithelium and bronchoalveolar lavage samples that are the starting material for the analyses of gene expression, carrying out immunohistochemical studies on the airway epithelial and alveolar macrophage populations to to assess the expression of selected genes at the morphologic level, analysis of bronchial biopsies to quantify the proportion of cell types to compare to the brushed samples, immunohistochemical analysis of the expression of selected genes, cell cycle analysis, analysis of the morphology of ciliated cells at the light microscopy level and of cilia perse using transmission electron microscopy, determination of cilia beat frequency, and quantitation of the extent of emphysema in the models of lung regeneration and to quantify the proportion of parenchymal cells contributed by circulating stem cells.

细胞生物学/形态学核心（核心D）提供全方位的生物成像服务 帮助COPD SCCOR研究者需要照片和显微照片文件 （可见光、荧光和电子）的基础和临床研究数据。除了成像 服务，核心提供其经验与样品处理和检测系统 （免疫组织化学、免疫荧光、原位杂交、扫描和透射电镜） 电子显微镜）分析实验样品中的基因和蛋白质表达。 核心小组的工作人员将与项目调查人员合作开发新的应用程序， 分析COPD SCORR中生成的数据。D核心捐款的具体例子 将包括协助项目量化大规模群体中的细胞类型， 以及作为起始材料的小气道上皮和支气管肺泡灌洗样品 为了分析基因表达，对气道进行免疫组织化学研究， 上皮细胞和肺泡巨噬细胞群，以评估在 形态学水平，分析支气管活检以量化细胞类型的比例， 与刷检标本比较，免疫组化分析所选 基因，细胞周期分析，光镜下纤毛细胞形态学分析 用透射电子显微镜观察纤毛本身的水平和纤毛搏动的测定 频率和肺再生模型中肺气肿程度的定量， 以量化由循环干细胞贡献的实质细胞的比例。