EFFECTS OF NICOTINE ON NPY FEEDING AND METABOLISM

尼古丁对 NPY 摄食和代谢的影响

• 批准号: 6013280
• 负责人: CHRISTOPHER R BISHOP
• 金额: $ 2.15万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-10 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6013280
• 关键词: Animalia; bioenergetics; eating; neuropeptide Y; nicotine; nicotinic receptors; nutrition related tag; paraventricular nucleus

While there is extensive information on the deleterious effects of smoking on health, approximately 26 percent of Americans continue to smoke. One of the factors mediating onset, continuance and relapse, particularly in women, is its weight-moderating effects. Though it has been demonstrated in animal models, that nicotine, the active ingredient in smoking, produces weight loss and subsequent weight gain following cessation of nicotine administration, mechanisms responsible for this effect have yet to be elucidated. This application proposes experiments designed to identify the role of a potent orexigenic compound, neuropeptide Y (NPY), in these effects. NPY is known to significantly potentiate food intake and biochemical utilization of carbohydrates in animals when injected into the paraventricular nucleus (PVN) of the hypothalamus, and has been recently shown to be altered during nicotine administration. In the proposed experiments, nicotine, administered via Alzet pumps, would be infused for 14 days followed by an abrupt cessation (pump explantation). The proposed set of experiments will identify the differential feeding, metabolic and gender effects of NPY on nicotine over acute, chronic and post-nicotine phases. These techniques will help to characterize the role of NPY in the weight-moderating effects of nicotine as well as how it affects food intake and metabolism.

尽管有大量关于吸烟对健康有害影响的信息，但大约 26% 的美国人仍然吸烟。 介导发病、持续和复发的因素之一，特别是在女性中，是其体重调节作用。 尽管已经在动物模型中证明，吸烟中的活性成分尼古丁会导致体重减轻，并在停止尼古丁给药后随后导致体重增加，但造成这种作用的机制尚未阐明。 本申请提出了一些实验，旨在确定有效的促食欲化合物神经肽 Y (NPY) 在这些作用中的作用。 众所周知，当注射到下丘脑的室旁核（PVN）时，NPY 可以显着增强动物的食物摄入和碳水化合物的生化利用，并且最近已被证明在尼古丁给药期间会发生改变。 在拟议的实验中，尼古丁通过 Alzet 泵注射，持续 14 天，然后突然停止（泵移植）。 拟议的一组实验将确定 NPY 在急性、慢性和尼古丁后阶段对尼古丁的不同喂养、代谢和性别影响。这些技术将有助于表征 NPY 在尼古丁体重调节作用中的作用，以及它如何影响食物摄入和新陈代谢。