EFFECTS OF NICOTINE ON NPY FEEDING AND METABOLISM

虽然有大量关于吸烟对健康有害影响的信息，但大约26%的美国人继续吸烟。调节发病、持续和复发的因素之一是它的减肥作用，特别是在女性中。虽然已经在动物模型中证明，吸烟的有效成分尼古丁在停止服用尼古丁后会产生体重减轻和随后的体重增加，但导致这种效果的机制尚未阐明。这项申请提出的实验旨在确定一种有效的促食欲素化合物神经肽Y(NPY)在这些效应中的作用。已知当NPY注射到下丘脑室旁核(PVN)时，显著增加动物的食物摄入量和碳水化合物的生物化学利用，最近被证明在尼古丁注射期间发生了改变。在拟议的实验中，通过老年痴呆症泵给药的尼古丁将被注入14天，然后突然停止(泵移植)。拟议的一组实验将确定NPY在急性、慢性和后尼古丁阶段对尼古丁的不同摄食、代谢和性别影响。这些技术将有助于确定NPY在尼古丁减肥效果中的作用，以及它如何影响食物的摄入和新陈代谢。

While there is extensive information on the deleterious effects of smoking on health, approximately 26 percent of Americans continue to smoke. One of the factors mediating onset, continuance and relapse, particularly in women, is its weight-moderating effects. Though it has been demonstrated in animal models, that nicotine, the active ingredient in smoking, produces weight loss and subsequent weight gain following cessation of nicotine administration, mechanisms responsible for this effect have yet to be elucidated. This application proposes experiments designed to identify the role of a potent orexigenic compound, neuropeptide Y (NPY), in these effects. NPY is known to significantly potentiate food intake and biochemical utilization of carbohydrates in animals when injected into the paraventricular nucleus (PVN) of the hypothalamus, and has been recently shown to be altered during nicotine administration. In the proposed experiments, nicotine, administered via Alzet pumps, would be infused for 14 days followed by an abrupt cessation (pump explantation). The proposed set of experiments will identify the differential feeding, metabolic and gender effects of NPY on nicotine over acute, chronic and post-nicotine phases. These techniques will help to characterize the role of NPY in the weight-moderating effects of nicotine as well as how it affects food intake and metabolism.