AMPHETAMINE INDUCED CHANGES IN BASAL GANGLIA OUTPUT

描述：(申请人摘要) 安非他明(Amph)引起特定物种的运动激活模式 在动物身上，已经证明至少部分是由 纹状体。如果纹状体中建立的神经元模式是 关键参与塑造对AMPH的运动反应，作为充足的数据 建议，那么这些模式的某些方面应该传达给它的 初级传出结构：实质黑质网状部(SNR)。 对这一应用的研究试图进一步了解黑质 Amph诱导运动反应的机制有两种。第一个是 探讨SNR在大鼠正常运动反应中的作用 在跑步机上行走或执行条件强化任务。 手术后植入微线束记录单个单位 在SNR中，大鼠将被放在有跑步机的房间里或有跑步机的房间里 两个鼻孔和一个用于液体蔗糖输送的喷嘴位于一个 墙。将在整个执行过程中监控单个单元的活动 这些任务应该会产生许多头部运动的例子，口头 行为(例如，舔)和运动。研究的第二个目的 在此提出的是将Amph诱导的SNR活性变化与 行为神经元在服药前的行为反应。演出结束后 在行为任务中，大鼠将被放置在开阔的野外环境中 在皮下感染后将监测单位的活动 安非他明(1.0或5.0 mg/kg)或生理盐水。这些实验将提供 关于SNR影响电机的方式的有价值的信息- 相关的目标结构，以及AMPH如何更改此输出以产生其 重复的或刻板印象的动作的特征模式。

DESCRIPTION: (Applicant's Abstract) Amphetamine (AMPH) elicits a species-specific pattern of motor activation that, in animals, has been shown to be mediated at least in part by the striatum. If the neuronal patters established in the striatum are critically involved in shaping the motor response to AMPH, as ample data suggest, then some aspect of these patterns should be communicated to its primary efferent structure the substantial nigra pars reticulate (SNr). Research in this application seeks to further understanding of the nigral mechanisms of AMPH-induced motor response in two ways. The first is to investigate the role of the SNr in the normal motor response of rats walking on a treadmill or performing a conditioned-reinforcement task. After surgery to implant microwire bundles for recording of single units in the SNr, rats will be placed in a chamber with a treadmill or one with two nose-poke holes and a spout for liquid sucrose delivery located on one wall. Single-unit activity will be monitored throughout performance of these tasks, which should yield numerous instances of head movements, oral behavior (e.g., licking) and locomotion. The second aim of the research proposed here is to correlate AMPH-induce changes in SNr activity and behavior the pre-drug behavioral response of the neuron. After performing the behavioral tasks, rats will be placed in an open-field environment where unit activity will be monitored after subcutaneous infections of either AMPH (1.0 or 5.0 mg/kg) or saline. These experiments will provide valuable information about the way in which the SNr influences motor- related target structures and how AMPH changes this output to produce its characteristic pattern of repetitive or stereotype movement.