Investigation of LA-MRSA in China and the UK

中国和英国LA-MRSA调查

• 批准号: MR/P007201/1
• 负责人: Mark Holmes
• 金额: $ 70.4万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FP007201%2F1
• 关键词: Investigation; LA; MRSA; China; UK

Both in China and the UK methicillin resistant Staphylococcus aureus (MRSA) remain an important problem that can lead to life threatening infections. A type of MRSA called livestock-associated MRSA (LA-MRSA) has been found both in China and the UK. While many strains of S. aureus seem to be restricted to particular host species, LA-MRSA are characterised by the fact that they freely jump between farm animals and people. The reservoir of LA-MRSA found on farms represents a considerable threat to human health and accounts for a large proportion of human MRSA infections in countries such as Denmark and the Netherlands. Recent reports from China show that the dominant LA-MRSA strains in China are different to those in Europe and North America. This research will determine the prevalence of LA-MRSA in China. Using whole genome sequencing, LA-MRSA found in people and animals will be compared to determine if these bacteria are transmitted from farms to people and also to look for genes that may be associated with the ability of LA-MRSA to colonise both people and animals. Farms where LA-MRSA are found will be compared to farms free of LA-MRSA to investigate any risk factors that may be associated with the presence of LA-MRSA. Further investigations of the host specificity of LA-MRSA will be performed using a technique called transposon mutagenesis. A library of many thousands of mutants will be made in LA-MRSA strains and used to identify individual genes that may play a role in enabling LA-MRSA avoid the immune defences of host animals and people.

在中国和英国，耐甲氧西林金黄色葡萄球菌 (MRSA) 仍然是一个可能导致危及生命的感染的重要问题。中国和英国都发现了一种被称为牲畜相关 MRSA (LA-MRSA) 的 MRSA。虽然金黄色葡萄球菌的许多菌株似乎仅限于特定的宿主物种，但 LA-MRSA 的特点是它们可以在农场动物和人类之间自由跳跃。农场中发现的 LA-MRSA 储存库对人类健康构成相当大的威胁，并且在丹麦和荷兰等国占人类 MRSA 感染的很大一部分。来自中国的最新报告显示，中国的主要LA-MRSA菌株与欧洲和北美的菌株不同。本研究将确定 LA-MRSA 在中国的流行情况。使用全基因组测序，对人和动物体内发现的 LA-MRSA 进行比较，以确定这些细菌是否从农场传播给人类，并寻找可能与 LA-MRSA 在人和动物中定殖的能力相关的基因。将发现 LA-MRSA 的农场与没有 LA-MRSA 的农场进行比较，以调查可能与 LA-MRSA 存在相关的任何风险因素。将使用转座子诱变技术对 LA-MRSA 的宿主特异性进行进一步研究。将在 LA-MRSA 菌株中建立一个包含数千个突变体的文库，并用于识别可能在使 LA-MRSA 避开宿主动物和人类的免疫防御方面发挥作用的单个基因。

项目成果

High-Throughput Mutagenesis Reveals a Role for Antimicrobial Resistance- and Virulence-Associated Mobile Genetic Elements in Staphylococcus aureus Host Adaptation.

• DOI: 10.1128/spectrum.04213-22
• 发表时间: 2023-02-23
• 期刊: Microbiology spectrum
• 影响因子: 3.7

Comparative Secretome Analyses of Human and Zoonotic Staphylococcus aureus Isolates CC8, CC22, and CC398.

• DOI: 10.1074/mcp.ra118.001036
• 发表时间: 2018-12
• 期刊: Molecular & cellular proteomics : MCP
• 作者: Busche T;Hillion M;Van Loi V;Berg D;Walther B;Semmler T;Strommenger B;Witte W;Cuny C;Mellmann A;Holmes MA;Kalinowski J;Adrian L;Bernhardt J;Antelmann H
• 通讯作者: Antelmann H

Antimicrobial resistance in human populations: challenges and opportunities.

• DOI: 10.1017/gheg.2017.4
• 发表时间: 2017
• 期刊: Global health, epidemiology and genomics
• 作者: Allcock S;Young EH;Holmes M;Gurdasani D;Dougan G;Sandhu MS;Solomon L;Török ME
• 通讯作者: Török ME

Simultaneously screening for methicillin-resistant Staphylococcus aureus and its susceptibility to potentiated penicillins.

同时筛查耐甲氧西林金黄色葡萄球菌及其对强化青霉素的敏感性。

• DOI: 10.1099/jmm.0.001562
• 发表时间: 2022
• 期刊: Journal of medical microbiology
• 影响因子: 3
• 作者: Ba X