ENDOTHELIUM AND HYPERTENSION AND PREGNANCY

内皮细胞与高血压和妊娠

• 批准号: 2857825
• 负责人: KENNETH E CLARK
• 金额: $ 25.28万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2857825
• 关键词: blood flow measurement; endothelin; enzyme linked immunosorbent assay; hemodynamics; high performance liquid chromatography; immunocytochemistry; kidney circulation; pregnancy circulation; pregnancy toxemia /hypertension; proteinuria; radioimmunoassay; receptor binding; respiratory gas analyzer; sheep; statistics /biometry; urinalysis; uterus; vascular endothelium; vascular resistance; vasoconstrictors

DESCRIPTION: (Adapted from the applicant's abstract) The objective of these experiments is to test the hypothesis that ET-1 plays an important role in regulating maternal systemic arterial blood pressure, systemic vascular resistance, uterine and renal hemodynamics, as well as renal permeability to proteins in nonpregnant and pregnant ewes. Aim 1 is to determine the dose of an ET-1 converting enzyme inhibitor required to block the conversion of big ET-1 to processed ET-1, and to determine if the endogenous production of ET-1 plays a role in modulating cardiovascular dynamics in pregnant and nonpregnant ewes. Aim 2 is to determine the systemic, uterine, and renal vascular responses to both local and intravenous infusion of ET-1. Aim 3 is to determine the mechanism by which the uterine artery becomes refractory to the vasoconstrictor actions of ET-1 during pregnancy. Aim 4 is to test the hypothesis that infusions of ET-1 results in hypertension, reduced uterine blood flow, hemoconcentration, and proteinuria in both pregnant and nonpregnant sheep.

描述：（改编自申请人的摘要） 实验是为了验证ET-1在 调节母体全身动脉血压，全身血管 阻力、子宫和肾脏血流动力学以及肾脏对 非妊娠和妊娠母羊中的蛋白质。 目的1是确定剂量 ET-1转化酶抑制剂，所需的阻断转化 大ET-1加工ET-1，并确定是否内源性生产的 ET-1在调节孕妇和新生儿心血管动力学中起作用， 未怀孕的母羊 目的2是确定全身、子宫和肾脏 血管对局部和静脉输注ET-1的反应。 目标3是 为了确定子宫动脉变得不应的机制， ET-1在妊娠期的血管收缩作用。 目标4是测试 假设ET-1的输注导致高血压、子宫收缩减少 血流量、血液浓缩和蛋白尿， 没有怀孕的羊

项目成果

Effect of Ro 61-1790, a selective endothelin-A receptor antagonist, on systemic and uterine hemodynamics and fetal oxygenation in sheep.

Ro 61-1790（一种选择性内皮素 A 受体拮抗剂）对绵羊全身和子宫血流动力学以及胎儿氧合的影响。

• DOI: 10.1067/mob.[phone]
• 发表时间: 2002
• 期刊: American journal of obstetrics and gynecology
• 影响因子: 9.8
• 作者: McElvy,Sherrie;Greenberg,Suzanne;Baker,RScott;Khoury,Jane;Clark,KennethE
• 通讯作者: Clark,KennethE