NOVEL MECHANISMS OF TROPHOBLAST GROWTH CONTROL

说明(改编自《调查员摘要》)：此修订版 应用建议研究细胞周期的调节 滋养层细胞分化前，这决定了可利用的 胎盘。具体地说，她建议1：建立关系 在细胞周期蛋白E表达、p21、p27和p57激酶活性水平与 免疫沉淀法和免疫印迹法检测正常胎盘发育 来自分离细胞制剂的分析；2：评估TGFb1的作用， 抑制滋养层细胞增殖，促进分化，在 细胞周期蛋白/抑制物比值与蛋白激酶活性及相关 细胞滋养层细胞分化；3：确定细胞滋养层细胞是否 可以通过操纵细胞周期蛋白：抑制物来改变分化程序 培养中的比例。

DESCRIPTION (Adapted from the Investigator's Abstract): This revised application proposes to investigate the regulation of the cell cycle in trophoblast cells prior to differentiation, which determines the available placenta. Specifically, she proposes to 1: establish the relationship between cyclin E expression, levels of p21, p27, and p57 kinase activity and normal placental development using immunoprecipitations and western blot analyses from isolated cell preparations; 2: evaluate effects of TGFb1, which inhibits trophoblast proliferation and promotes differentiation, on the cyclin/ inhibitor ratios and kinase activity and associated cytotrophoblast differentiation; 3: determine if the cytotrophoblast differentiation program can be altered by manipulating the cyclin:inhibitor ratio in culture.