REALTIME PCR INSTRUMENTATION

实时 PCR 仪器

• 批准号: 6292190
• 负责人: JULIE A DELOIA
• 金额: $ 9.53万
• 依托单位: MAGEE-WOMEN'S HOSPITAL OF UPMC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292190
• 关键词: biomedical equipment; biomedical equipment purchase; nucleic acid sequence; polymerase chain reaction

The quantitative assessment of gene expression is a cornerstone of modern biology. The advent of real-time PCR has tremendously facilitate this process by providing greater accuracy and time savings, as well as reducing the risk of communication. The goal of this application is to obtain funding for real-time PCR instrumentation within the Agee- Women Research Institute. Over the past 8 years, the MWRI has grown substantially in the number of investigators and NIH funding. Currency, MWRI members hold 31 NIH-funded grants totaling over $7,300,000 per year. We have a cadre of research using cutting-edge molecular biology techniques. Unfortunately, researchers frequently must travel to other university laboratories for the necessary instrumentation or use older technology to conduct their research. With this explosion of researchers and projects within the MWRI, we now have a need for immediate access to real-time PCR. Most of the investigators at MWRI will use the instrumentation to candidate differences in gene expression in various research paradigms related to pregnancy, development, infections, and in vitro modeling. Both human and animal tissues will be analyzed. In addition, direct genomic analyses such as determination of gene copy number will be preformed in ovarian cancers that are resistant to chemotherapy. Another application will be analyses of genetic polymorphism in women with preeclampsia. Members of the departments of Obstetrics, Gynecology and Reproductive Sciences, Pediatrics and Pathology are represented in this application. Thus, the proposal real-time PCR machine would be core facility utilized by many NIH- funded researchers.

基因表达的定量评估是现代生物学的基石。实时聚合酶链式反应的出现极大地促进了这一过程，因为它提供了更高的准确性和节省的时间，以及降低了沟通的风险。该应用程序的目标是为AGEE妇女研究所内的实时聚合酶链式反应仪器获得资金。在过去的8年里，MWRI在调查人员的数量和NIH的资金方面都有了实质性的增长。货币方面，MWRI成员每年拥有31笔NIH资助的赠款，总额超过7300,000美元。我们有一批使用尖端分子生物学技术的研究人员。不幸的是，研究人员经常必须前往其他大学实验室进行必要的仪器测试，或者使用较老的技术进行研究。随着MWRI内部研究人员和项目的激增，我们现在需要立即获得实时聚合酶链式反应。MWRI的大多数研究人员将使用该仪器来评估与怀孕、发育、感染和体外建模相关的各种研究范式中基因表达的差异。将对人类和动物组织进行分析。此外，还将对化疗耐药的卵巢癌进行直接的基因组分析，如确定基因拷贝数。另一个应用将是对先兆子痫妇女的基因多态性进行分析。产科、妇科和生殖科学系、儿科和病理学系的成员在本申请中有代表。因此，提议的实时PCR机将成为许多NIH资助的研究人员使用的核心设施。