CHEMICAL RELEASE & IMMUNE SENSITIZATION BY DENTAL RESINS

化学释放

• 批准号: 2897033
• 负责人: WYATT R HUME
• 金额: $ 18.3万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2897033
• 关键词: apoptosis; biopsy; biotransformation; chemical hypersensitivity; clinical research; composite resins; contact dermatitis; delayed hypersensitivity; dental pulp; dentin; drug adverse effect; excretion; fluorescence microscopy; guinea pigs; human subject; immune tolerance /unresponsiveness; immunocytochemistry; in situ hybridization; laboratory mouse; oral facial restoration material; oral mucosa; polymerase chain reaction; polymethacrylate; skin hypersensitivity; tissue /cell culture

DESCRIPTION (Adapted from investigator's Abstract): This is a competing continuation application whose overall goal is to ensure that resin-based materials which are placed onto teeth as part of dental care can continue to be used safely. Resin based materials which polymerize in the mouth are now used by dentists in many ways to help prevent tooth decay and for tooth repair and replacement. Bonding technologies, which are all based on this class of materials, are used for fissure sealing to help prevent decay, for bonding orthodontic brackets to teeth, and as part of many different types of tooth repair to improve appearance and function. It is very likely that new and modified materials of the same general type will continue to be developed and that the use of this type of material in dentistry will continue to increase in the coming years. The materials are very helpful to patients and have remarkably few negative side effects. However, severe allergic dermatitis in some dentists and other dental workers is linked to the use of the materials, and the incidence of such allergy appears to be increasing. Fortunately, allergic responses are less common in patients, but we do not know why this is so or whether it will continue to be so. Some patients experience pain in the dental pulp after resins are used in deep fillings, for reasons that may be related to direct chemical damage caused by released chemicals, by allergy to them, or to both. It has previously been shown in laboratory studies that two chemicals are released from these materials during the first days after they are placed on teeth. The first aim will be to confirm this release in experimental animals (guinea pigs), and study what happens to the chemicals in the body (their uptake, distribution, time of storage, breakdown and excretion). The second aim will be to study mechanisms of allergic responses to these chemicals at the cellular and molecular level using guinea pigs and mice. The third aim will be to determine whether there are differences between the risk of allergy with skin contact (as can occur in dental workers) relative to contact with the inside of the mouth and through tooth structure to the tooth pulp (as can occur in patients) in the same animals. The fourth aim will be to study allergic responses at the cellular and molecular level using blood and other tissues donated by volunteer dentists and other dental workers, to ensure that the experimental studies of the phenomenon using animal models and animal tissues in culture are valid. The studies described will help to prevent and treat adverse effects of this class of dental materials.

描述(改编自《调查员摘要》)：这是一场竞争 继续应用，其总体目标是确保基于树脂的 作为牙齿护理的一部分，放置在牙齿上的材料可以继续 安全使用。在口腔中聚合的树脂基材料现在是 牙医在许多方面用来帮助防止龋齿和牙齿 维修和更换。粘合技术，这些技术都是基于 一类材料，用于缝隙密封以帮助防止腐蚀， 将正畸托槽粘接到牙齿上，并作为许多不同类型的一部分 牙齿修复，以改善外观和功能。很有可能是 相同通用类型的新材料和改性材料将继续 而且这种材料在牙科中的使用将会 在接下来的几年里继续增加。这些材料对……很有帮助 患者，并且几乎没有负面副作用。然而，严重的 一些牙医和其他牙科工作者的过敏性皮炎与 材料的使用，以及这种过敏的发生率似乎是 越来越多。幸运的是，过敏反应在患者中不太常见， 但我们不知道为什么会这样，也不知道这种情况是否会继续下去。 一些患者在使用树脂治疗后会感到牙髓疼痛 深层填充物，原因可能与直接化学损害有关 由释放的化学物质引起，或对其过敏，或两者兼而有之。它有 以前的实验室研究表明，有两种化学物质被释放出来 在这些材料被放置在牙齿上后的第一天。 第一个目标是在实验动物身上证实这一释放。 (豚鼠)，并研究体内的化学物质(它们的 摄取、分配、储存时间、分解和排泄)。第二 目的是研究对这些化学物质的过敏反应机制 用豚鼠和小鼠进行细胞和分子水平的研究。第三个目标 将确定风险之间是否存在差异 皮肤接触过敏(可能发生在牙科工作人员中) 与口腔内侧接触，并通过牙齿结构到达 同一种动物的牙髓(患者也可能发生这种情况)。第四个目标 将在细胞和分子水平上研究过敏反应 使用志愿牙医和其他牙科医生捐赠的血液和其他组织 工作人员，以确保实验研究的现象使用 培养的动物模型和动物组织是有效的。这些研究 所描述的将有助于预防和治疗这类疾病的不良影响 牙科材料。