CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
批准号:
6296774
负责人:
BERTIL HILLE
金额:
$16.65万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-04-01 至 2000-03-31
关键词:
calcium flux exocytosis follicle stimulating hormone gonadotropin releasing factor gonadotropins hormone regulation /control mechanism inositol phosphates luteinizing hormone membrane activity reproductive hormone single cell analysis spectrometry sperm tissue /cell culture voltage /patch clamp western blottings
中文摘要
目的是填补我们在电生理方面的空白
英文摘要
The goal is to fill in gaps concerning our electrophysiological
understanding of GnRH neurons and gonadotropes in order to learn how they
are stimulated to secrete and if they have intrinsic mechanisms that would
explain the rhythmicity and pulse shape of secretion. Three cell types
will be used initially: a neuron-like GnRH-secreting cell line derived
from a transgenic mouse tumor; a gonado trope-like alphaT-3 cell line
derived from a transgenic mouse pituitary tumor; and juvenile and adult
gonadotropes enzymatically dissociated from salmon anterior pituitaries.
Responses will be studied primarily by membrane biophysical techniques
including: whole-cell and patch clamp methods to characterize the ionic
channels and their response to GnRH in these cells; changes of membrane
capacitance as an index of exocytosis of secretory vesicles; and
intracellular calcium changes with indo 1 fluorescent dye. The specific
aims are: 1) To inventory the voltage-gated ionic channels and to
determine the electrical activity they can support. 2) To inventory the
fast ligand-gated channels. 3) To determine the actions of GnRH on
channels, intracellular calcium, and secretion. 4) To determine modulatory
actions of other peptides and neurotransmitters acting through G-protein-
coupled receptors. 5) To explore the intracellular signaling pathways used
for responses to GnRH and other modulatory transmitters. 6) To test
hypotheses regarding the electrophysiological basis of excitation,
inhibition, and ultradian rhythmicity.
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MODULATION OF ION CHANNELS BY PHOSPHOINOSITIDE METABOLISM
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批准号:8362510
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项目类别:
-
资助金额:$1.05万
-
财政年份:2011
-
负责人:BERTIL HILLE
-
依托单位:
MODULATION OF ION CHANNELS BY PHOSPHOINOSITIDE METABOLISM
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批准号:8169583
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项目类别:
-
资助金额:$1.63万
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财政年份:2010
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负责人:BERTIL HILLE
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依托单位:
INTRACELLULAR DYNAMICS OF CALCIUM SIGNALS AND EXOCYTOSIS
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批准号:7956413
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项目类别:
-
资助金额:$1.63万
-
财政年份:2009
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负责人:BERTIL HILLE
-
依托单位:
MODULATION OF ION CHANNELS BY PHOSPHOINOSITIDE METABOLISM
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批准号:7956412
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项目类别:
-
资助金额:$1.63万
-
财政年份:2009
-
负责人:BERTIL HILLE
-
依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:7553382
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项目类别:
-
资助金额:$40.55万
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财政年份:2007
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负责人:BERTIL HILLE
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依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6588487
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项目类别:
-
资助金额:$15.33万
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财政年份:2002
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负责人:BERTIL HILLE
-
依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6655307
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项目类别:
-
资助金额:$15.33万
-
财政年份:2002
-
负责人:BERTIL HILLE
-
依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6311615
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项目类别:
-
资助金额:$16.65万
-
财政年份:2000
-
负责人:BERTIL HILLE
-
依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6108321
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项目类别:
-
资助金额:$16.65万
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财政年份:1999
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负责人:BERTIL HILLE
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依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6272021
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项目类别:
-
资助金额:$16.72万
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财政年份:1998
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负责人:BERTIL HILLE
-
依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6240876
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项目类别:
-
资助金额:$14.24万
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财政年份:1997
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负责人:BERTIL HILLE
-
依托单位:
CONTROL OF CELLS OF THE REPRODUCTIVE AXIS
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批准号:6444008
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项目类别:
-
资助金额:$15.33万
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财政年份:1996
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负责人:BERTIL HILLE
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依托单位:
PROTEIN OF EXCITABLE MEMBRANE: A SYMPOSIUM
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批准号:3436032
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项目类别:
-
资助金额:$1.0万
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财政年份:1985
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负责人:BERTIL HILLE
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依托单位:
ELECTRIC STUDIES OF EXCITATION, SECRETION & CONTRACTION
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批准号:3154962
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项目类别:
-
资助金额:$19.88万
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财政年份:1977
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负责人:BERTIL HILLE
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依托单位:
Electric Studies of Excilation, Secretion & Contraction
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批准号:6729947
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项目类别:
-
资助金额:$28.5万
-
财政年份:1977
-
负责人:BERTIL HILLE
-
依托单位:
Electric Studies of Excitation, Secretion & Contraction
-
批准号:7215636
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项目类别:
-
资助金额:$24.87万
-
财政年份:1977
-
负责人:BERTIL HILLE
-
依托单位:
Electric Studies of Excitation, Secretion & Contraction
-
批准号:6573456
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项目类别:
-
资助金额:$28.5万
-
财政年份:1977
-
负责人:BERTIL HILLE
-
依托单位:
ELECTRIC STUDIES OF EXCITATION, SECRETION & CONTRACTION
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批准号:2899827
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项目类别:
-
资助金额:$22.49万
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财政年份:1977
-
负责人:BERTIL HILLE
-
依托单位:
Electric studies of excitation, secretion & contraction
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批准号:9896832
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项目类别:
-
资助金额:$38.03万
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财政年份:1977
-
负责人:BERTIL HILLE
-
依托单位:
Electric Studies of Excitation, Secretion & Contraction
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批准号:7368352
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项目类别:
-
资助金额:$34.32万
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财政年份:1977
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负责人:BERTIL HILLE
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依托单位:
海外基金