Electric studies of excitation, secretion & contraction

兴奋、分泌的电学研究

• 批准号: 9896832
• 负责人: BERTIL HILLE
• 金额: $ 38.03万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9896832
• 关键词: Acetylcholine; Acute; Address; Adrenergic Receptor; Affective; Anatomy; Area; Arrestins; Arylalkylamine N-Acetyltransferase; Attention; Biochemical Genetics; Biophysics; Blood specimen; Brain; Calcium; Calcium Signaling; Cell Culture Techniques; Cell Line; Cell physiology; Cells; Clinical; Coupled; Coupling; Cyclic AMP; Disease; Dyes; Electrophysiology (science); Endocrine Glands; Enzymes; Exposure to; Flow Cytometry; Fluorescence Microscopy; Funding; G-Protein-Coupled Receptors; GTP-Binding Proteins; Gene Expression; Gland; Glutamates; Heterogeneity; High Pressure Liquid Chromatography; Hormone secretion; Hormones; Hour; Hydrolysis; Ion Channel; Jet Lag Syndrome; Kinetics; Maps; Mass Spectrum Analysis; Mediating; Mediator of activation protein; Melatonin; Melatonin Receptors; Membrane; Messenger RNA; Microscope; Nerve; Nerve Endings; Neurons; Neuropeptides; Neurotransmitters; Norepinephrine; Norepinephrine Receptors; Periodicity; Pharmacologic Substance; Phosphatidylinositols; Phospholipids; Photometry; Physiology; Pineal gland; Pinealocyte; Productivity; Published Comment; Rattus; Receptor Signaling; Regulation; Second Messenger Systems; Serotonin; Side; Signal Transduction; Sleep Disorders; Slice; Solvents; Source; Sympathetic Nervous System; System; Techniques; Testing; Thinking; Time; Transferase; autocrine; cell type; circadian; circadian pacemaker; circadian regulation; desensitization; gamma-Aminobutyric Acid; immunocytochemistry; indoleamine; operation; reconstitution; response; shift work; synthetic enzyme

The pineal gland is an endocrine organ that is part of our circadian clock system in the brain. Throughout the night, noradrenaline released locally by the sympathetic nervous system stimulates the pineal to broadcast the night hormone, melatonin, to all of the body. We will study the early second-messenger signaling within isolated pinealocytes in response to applied norepinephrine, the longer term availability of adrenergic receptors during prolonged exposure to norepinephrine, and, in pineal slices and whole pineal glands, the modulatory actions of classical neurotransmitters on pinealocyte activity. We will determine the G-protein coupling of melatonin receptors expressed in various expression systems. Finally we will test the hypothesis that N-acetyl serotonin is a candidate for another night hormone in addition to melatonin. Cells will be dissociated from rat pineal glands, placed in cell culture, treated with norepinephrine to mimic night, and studied under the microscope with techniques such as whole-cell gigaseal electrophysiological recording, live-cell photometry of calcium-sensitive dyes and of indicators for second messengers, and HPLC-mass spectroscopy of the three indoleamine hormones. Solvent extracts of whole pineal glands will be analyzed by HPLC-mass spectroscopy for the phosphoinositides and other phospholipids of their membranes. Under- standing the rhythmic secretory mechanisms of the pineal will make an important contribution towards treating sleep disorders, seasonal affective responses to short days, and loss of attention and productivity due to jet lag and shift work.

松果体是一个内分泌器官，是我们大脑中生物钟系统的一部分。 整晚，交感神经系统局部释放去甲肾上腺素 刺激松果体向全身传播夜间荷尔蒙--褪黑素。我们会研究 松果体细胞内早期第二信使信号对外加的响应 去甲肾上腺素，肾上腺素能受体在长期暴露中的长期可获得性 去甲肾上腺素，以及在松果体切片和整个松果体中， 经典神经递质对松果体细胞活性的影响。我们将测定G蛋白偶联。 褪黑素受体在不同的表达系统中表达。最后，我们将测试 假设N-乙酰-5-羟色胺是另一种夜间激素的候选者 褪黑激素。将细胞从大鼠松果腺中分离出来，置于细胞培养中，用 去甲肾上腺素模拟夜晚，并在显微镜下进行研究，技术包括 钙敏感染料的全细胞千兆脑电生理记录和活细胞测光 和第二信使的指示物，以及三种物质的高效液相色谱-质谱学 吲哚胺激素。整个松果体的溶剂提取物将用高效液相色谱-质谱仪进行分析。 肌醇磷脂及其膜上的其他磷脂的光谱。在- 站在松果体节律性分泌机制上将做出重要贡献 对睡眠障碍的治疗，对短白天的季节性情感反应，以及 由于时差和倒班工作而引起的注意力和工作效率。

项目成果

Control of granule mobility and exocytosis by Ca2+ -dependent formation of F-actin in pancreatic duct epithelial cells.

• DOI: 10.1111/j.1600-0854.2009.00884.x
• 发表时间: 2009-04
• 期刊: Traffic (Copenhagen, Denmark)
• 作者: Jung SR;Kim MH;Hille B;Koh DS
• 通讯作者: Koh DS

A fast solution switching system with temperature control for single cell measurements.

用于单细胞测量的带温度控制的快速溶液切换系统

• DOI: 10.1016/j.jneumeth.2011.04.023
• 发表时间: 2011-07-15
• 期刊: JOURNAL OF NEUROSCIENCE METHODS
• 影响因子: 3
• 作者: Koh, Duk-Su;Chen, Liangyi;Ufret-Vincenty, Carmen A.;Jung, Seung-Ryoung
• 通讯作者: Jung, Seung-Ryoung

GABAergic signaling in the rat pineal gland.

• DOI: 10.1111/jpi.12328
• 发表时间: 2016-08
• 期刊: Journal of pineal research
• 影响因子: 10.3
• 作者: Yu H;Benitez SG;Jung SR;Farias Altamirano LE;Kruse M;Seo JB;Koh DS;Muñoz EM;Hille B
• 通讯作者: Hille B

Glutamate transporter-mediated glutamate secretion in the mammalian pineal gland.

哺乳动物松果体中的谷氨酸转运蛋白介导的谷氨酸分泌。

• DOI: 10.1523/jneurosci.0894-08.2008
• 发表时间: 2008-10-22
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Kim MH;Uehara S;Muroyama A;Hille B;Moriyama Y;Koh DS
• 通讯作者: Koh DS

Cyclic AMP potentiates Ca2+-dependent exocytosis in pancreatic duct epithelial cells.

• DOI: 10.1085/jgp.200910355
• 发表时间: 2010-05
• 期刊: The Journal of general physiology
• 作者: Jung SR;Hille B;Nguyen TD;Koh DS
• 通讯作者: Koh DS