TRANSMISSION AND GENETICS OF ALCOHOL DISORDERS IN A NATIVE AMERICAN TRIBE

美洲原住民部落中酒精疾病的传播和遗传学

• 批准号: 6288631
• 负责人: Jeffrey C. LONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6288631
• 关键词: Native Americans; alcoholism /alcohol abuse; clinical research; family genetics; gene environment interaction; genetic polymorphism; genetic susceptibility; genome; genotype; human subject; interview; linkage mapping; mental health epidemiology; nucleic acid repetitive sequence

Using a whole genome scan, we will assess genetic linkage to alcoholism and associated psychiatric disorders in Choctaw American Indians. Choctaw is a large Eastern North American Indian tribe with over 30,000 enrolled members living within tribal boundaries in Oklahoma. By contrast to neighboring American Indian tribes that have high prevalences of alcoholism, this tribe stands out because alcoholism has a low prevalence, about 1% and 10% of females and males, respectively. By studying American Indians in the context low alcoholsim, we can expect to reveal different insights into the roles of genetic and/or environmental determinants of alcoholism. Genetic analysis is to be conducted using three samples from the tribe: a small random sample (N=100), 3 large extended families (N>80 per family), and a sample of more than 150 admixed nuclear families. The admixed nuclear familes will be selected to have some Euro-american ancestry and at least one alcoholic family member. The transmission/disequilibrium test (TDT) is the linkage analysis method of principal interest, because it has been shown to have increased power with population admixture. However, the sampling design will also accomodate standard non-parametric two- and multi-point linkage methods. In order to perform the analyses outlined above, individual psychiatric interviews and blood samples have been and continue to be collected. Research diagnoses are made from the psychiatric interviews and DNA for genotyping is being extracted from the blood samples. For the linkage analysis, we expect to type up to 2000 unique sequence DNA polymorphisms spanning the entire human genome as the technology becomes available. In the past year, collection of the random sample and extended families has been completed. Database checks and epidemiological analyses are now under way. - gene mapping (human), neurosciences, drinking patterns/causes, molecular genetics - Human Subjects: Interview, Questionaires, or Surveys Only

使用全基因组扫描，我们将评估遗传联系，酗酒和相关的精神疾病的乔托美洲印第安人。乔托是北美东部的一个大型印第安部落，有超过30，000名注册成员居住在俄克拉荷马州的部落边界内。与邻近的酗酒率很高的美洲印第安部落相比，这个部落之所以突出，是因为酗酒率很低，女性和男性分别约为1%和10%。通过研究美国印第安人的背景下低酒精，我们可以期望揭示不同的见解的遗传和/或环境的决定因素的作用，酗酒。遗传分析将使用来自部落的三个样本进行：一个小的随机样本（N=100），3个大的大家庭（每个家庭N>80），以及一个超过150个混合核心家庭的样本。混合的核心家庭将被选择有一些欧美血统和至少一个酗酒的家庭成员。传递/不平衡检验（transmission/disequilibrium test，TDT）是主要利益的连锁分析方法，因为它已被证明具有增加的功效与人口混合。然而，抽样设计也将适应标准的非参数两点和多点连锁方法。为了进行上述分析，已经并将继续进行个别精神病访谈和收集血液样本。研究诊断是从精神病访谈中作出的，基因分型的DNA是从血液样本中提取的。对于连锁分析，我们希望在技术成熟时，能够对跨越整个人类基因组的多达2000个独特序列DNA多态性进行分型。在过去一年中，已经完成了随机抽样和大家庭的收集工作。目前正在进行数据库检查和流行病学分析。- 基因图谱（人类），神经科学，饮酒模式/原因，分子遗传学-人类主题：访谈，问卷调查或调查