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Unravelling post-transcriptional regulatory networks in pathogenic S. aureus

解开致病性金黄色葡萄球菌的转录后调控网络

基本信息

批准号：
MR/R008205/1
负责人：
Sander Granneman
金额：
$ 241.19万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Fellowship
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=MR%2FR008205%2F1
关键词：
Unravelling post transcriptional regulatory networks

项目摘要

Antimicrobial medicines have saved millions of lives since the introduction of penicillin in the 1940s. But their overuse has resulted in the rise of multidrug-resistant bacteria at a rate that has outpaced the discovery of new antibiotics. The emergence of multi-drug resistant Staphylococcus aureus (such as MRSA) in particular is causing major healthcare problems world-wide as S. aureus skin and respiratory infections can be life-threatening and are becoming increasingly more difficult to treat. Like all organisms, MRSA initially makes temporary copies of its genes, called messenger RNA (mRNA) molecules, which can subsequently be read, or translated, by a molecular machine called the ribosome to generate proteins. How much of a protein is made depends on how much of its mRNA is present and how well it accesses the translation machinery. Proteins enable the organism to survive and, for bacteria like S. aureus, to infect human cells. One reason why S. aureus is such a successful human pathogen is because it can quickly remove mRNAs that are no longer required and control which mRNAs are translated. This enables the organism to swiftly adapt to challenges from the immune system by changing the proteins that it makes. This mechanism is crucial for S. aureus survival during infection, but we know remarkably little about how it works. The goal of our research is to gain detailed molecular insights into this process, using innovative techniques that we have developed over the years. The results from our studies may help uncover new ways of battling infectious diseases.

自20世纪40年代青霉素问世以来，抗菌药物已经挽救了数百万人的生命。但它们的过度使用导致了耐多药细菌的增加，其速度超过了新抗生素的发现。尤其是多重耐药金黄色葡萄球菌（如MRSA）的出现，在世界范围内引起了重大的医疗保健问题，如S。金黄色葡萄球菌皮肤和呼吸道感染可能危及生命，并且变得越来越难以治疗。像所有生物体一样，MRSA最初会暂时复制其基因，称为信使RNA（mRNA）分子，随后可以通过称为核糖体的分子机器读取或翻译以产生蛋白质。一种蛋白质的合成量取决于它的mRNA的含量以及它与翻译机制的连接程度。蛋白质使有机体得以生存，对于像S.金黄色葡萄球菌，感染人类细胞。S.金黄色葡萄球菌是一种如此成功的人类病原体，因为它可以快速去除不再需要的mRNA并控制哪些mRNA被翻译。这使生物体能够通过改变其制造的蛋白质来迅速适应免疫系统的挑战。这一机制对S.金黄色葡萄球菌在感染过程中存活，但我们对其如何工作知之甚少。我们研究的目标是利用我们多年来开发的创新技术，获得对这一过程的详细分子见解。我们的研究结果可能有助于发现对抗传染病的新方法。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Puf6 primes 60S pre-ribosome nuclear export at low temperature.

DOI：
10.1038/s41467-021-24964-2
发表时间：
2021-08-04
期刊：
Nature communications
影响因子：
16.6
作者：
Gerhardy S;Oborská-Oplová M;Gillet L;Börner R;van Nues R;Leitner A;Michel E;Petkowski JJ;Granneman S;Sigel RKO;Aebersold R;Panse VG
通讯作者：
Panse VG

Advantages and Limitations of UV Cross-linking Analysis of Protein-RNA Interactomes in Microbes

微生物中蛋白质-RNA 相互作用组的紫外交联分析的优点和局限性

DOI：
10.22541/au.167513944.47153233/v1
发表时间：
2023
期刊：
影响因子：
0
作者：
Granneman S
通讯作者：
Granneman S

Temporal-iCLIP captures co-transcriptional RNA-protein interactions.

DOI：
10.1038/s41467-023-36345-y
发表时间：
2023-02-08
期刊：
NATURE COMMUNICATIONS
影响因子：
16.6
作者：
Cordiner, Ross A.;Dou, Yuhui;Thomsen, Rune;Bugai, Andrii;Granneman, Sander;Heick Jensen, Torben
通讯作者：
Heick Jensen, Torben

Bacterial Regulatory RNA - Methods and Protocols

细菌调节 RNA - 方法和实验方案

DOI：
10.1007/978-1-0716-3565-0_17
发表时间：
2024
期刊：
影响因子：
0
作者：
Esteban-Serna S
通讯作者：
Esteban-Serna S

pyRBDome: A comprehensive computational platform for enhancing and interpreting RNA-binding proteome data

DOI：
10.1101/2023.12.08.570608
发表时间：
2023-12
期刊：
bioRxiv
影响因子：
0
作者：
Liang-Cui Chu;Niki Christopoulou;Hugh McCaughan;Sophie Winterbourne;Davide Cazzola;Shichao Wang;Ulad Litvin;Salomé Brunon;Patrick J.B. Harker;Iain McNae;S. Granneman
通讯作者：
Liang-Cui Chu;Niki Christopoulou;Hugh McCaughan;Sophie Winterbourne;Davide Cazzola;Shichao Wang;Ulad Litvin;Salomé Brunon;Patrick J.B. Harker;Iain McNae;S. Granneman