ELECTROPORATION SYSTEM FOR ADHERENT CELLS

在研究实验室中，使用电放电对悬浮液和贴壁细胞进行电击穿的情况正在增长。然而，用于电穿孔电池的传统工艺不容易适应不同的格式或大容量产量。拟议的电穿孔贴壁细胞工艺有望扭转这一趋势。新的、低成本的材料将被用来建造电穿孔室，这种电穿孔室可以很容易地适应从96孔培养皿到大型生物反应器的许多不同形式。拟议的商业应用：拟议的研究将导致开发几种用于进行电穿孔实验的新产品。这些产品将被设计成与现有的电穿孔电源接口，预计将满足对更高实用性和更低成本产品的需求。

The use of electrical discharges in research laboratories for electroprating suspension and adherent cells is growing. Conventional processess for electroporating cells, however, are not readily adapted to different formats or high volume throughput. The proposed process for electroporating adherent cells promises to reverse this trend. New, low cost materials will be used to construct electroporation chambers that can be easily adapted to many different formats ranging from 96-well culture dishes to large-scale bioreactors. PROPOSED COMMERCIAL APPLICATION: The proposed research will lead to the development of several new products for performing electroporation experiments. The products which will be designed to interface with existing electroporation power supplies, are anticipated to fill the need for increased utility and lower cost products.