NOVEL HIGHTHROUGHOUT SCREENING ASSAY

新颖的高通量筛选试验

• 批准号: 6016532
• 负责人: EDWARD M DAVIS
• 金额: $ 10万
• 依托单位: SYMBIOTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-07 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6016532
• 关键词: antigen antibody reaction; biological signal transduction; biomedical equipment development; drug discovery /isolation; enzyme inhibitors; enzyme substrate; high throughput technology; intermolecular interaction; photosynthesis; protein tyrosine kinase; receptor binding; technology /technique development

DESCRIPTION: (adapted from applicant's abstract) The aim of this SBIR proposal is to develop a novel, rapid, homogeneous assay for high throughput screening which is capable of detecting affinity interactions between antibody/antigen, receptor/ligand, and enzyme/substrate. High throughput screening (HTS) assays based on this new technology will have applications in pharmaceutical and biotechnology combinatorial drug discovery programs. The specific aims will be to develop novel reagents used in the assay, optimize assay conditions and develop instrumentation for performing the assay. The HTS assay combines the use of specially formulated photosynthetic membrane and fluorescence detection. The proposed work will focus on developing an HTS assay for identification of inhibitors of protein tyrosine kinases involved in signal transduction. An assay will be developed to identify inhibitors of src, an oncogenic protein tyrosine kinase overexpressed in certain cancer cells. PROPOSED COMMERCIAL APPLICATION: The high throughput screening assay has commercial applications in drug discovery programs and will be marketed to pharmaceutical and biotechnology companies. The technology can also be developed for application in vitro diagnostic assays for use in the biomedical, food, dairy and environmental industries.

描述：(改编自申请人的摘要)本SBIR提案的目的 是为高通量筛选开发一种新的、快速的、均一的检测方法 它能够检测抗体/抗原之间的亲和力相互作用， 受体/配体和酶/底物。高通量筛选(HTS)分析 基于这一新技术将在制药和 生物技术组合药物发现计划。具体目标将是 开发用于检测的新试剂，优化检测条件，并 开发用于执行分析的仪器。HTS检测结合了 使用特殊配方的光合膜和荧光检测。 拟议的工作将集中于开发一种HTS检测方法，用于鉴定 蛋白酪氨酸激酶的抑制物参与信号转导。一个 将开发一种检测方法来鉴定src的抑制剂，src是一种致癌蛋白 酪氨酸激酶在某些癌细胞中过度表达。 建议的商业应用： 高通量筛选试验在药物发现中有商业应用 计划，并将向制药和生物技术公司营销。这个 也可以开发用于体外诊断分析的技术 在生物医药、食品、乳制品和环境行业。