SOUTHERN BLOTTING FOLLOWING CAPILLARY ELECTROPHORESIS

毛细管电泳后进行印迹印迹

• 批准号: 3498683
• 负责人: EDWARD M DAVIS
• 金额: $ 4.72万
• 依托单位: SYMBIOTECH, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-18 至 1992-01-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498683
• 关键词: gel; gel electrophoresis; hollow fiber separation technique; restriction fragment length polymorphism; southern blotting; technology /technique; technology /technique development

DESCRIPTION (Adapted from applicant's abstract): Capillary electrophoresis is becoming an important analytical tool. Recent results show that DNA sequencing can be performed with capillary gel electrophoresis. To further increase the utility of this analytical procedure, it is proposed to develop a new process for transferring DNA and other analytes to nylon membranes by blotting. This process will have wide application including restriction fragment length polymorphism analysis and multiplex DNA sequencing.

描述（改编自申请人摘要）：毛细管电泳 正在成为一种重要的分析工具。 最近的研究表明，DNA 可以用毛细管凝胶电泳进行测序。 进一步 为了提高该分析方法的实用性，建议 开发将DNA和其他分析物转移到尼龙上的新工艺 膜通过印迹。 该方法将具有广泛的应用，包括 限制性片段长度多态性分析和多重DNA 测序