3DBioNet: an integrated technological platform for 3D micro-tissues

3DBioNet：3D微组织集成技术平台

• 批准号: MR/R025762/1
• 负责人: Raphael Levy
• 金额: $ 79.77万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FR025762%2F1
• 关键词: 3DBioNet; integrated; technological; platform; 3D

Maybe you have already seen cells under a microscope? Plants, animals and humans are made of cells. For approximately a century, scientists have routinely grown cells on flat transparent surfaces (glass or plastic). Growing cells on flat surfaces is very convenient. In fact, it has been so successful that a lot of what we know about cell components such as the nucleus (which contains the genetic information) and mitonchodria (which provides energy) comes from such experiments; this also includes discovery of drugs and tests of their potential toxicity. However, in real living organisms cells are not attached to a flat surface. Instead, they interact with their three dimensional environment which includes other cells as well as factors secreted by cells. Scientists now realize more and more the importance of this difference between growing cells on a flat surface or growing them in 3D: it modifies their shape, how they communicate with each others and how they respond to changes in their environment. Until recently, animal experiments have been the main alternative to cells grown on flat surfaces. Whilst animal experiments remain essential to our understanding of biology and to the discovery of new drugs, they do not perfectly mimic human biology and it is also desirable to reduce their number for ethical reasons.In the last decade, scientists have started to culture cells in 3D thereby producing micro-tissues, often starting from stem cells. These micro-tissues made of human-derived cells resemble human tissues. But, just like going from 2D printing to 3D printing requires new materials, new software, new ideas and new procedures, going from 2D cell culture to 3D cell culture requires a large number of innovations in the methods, materials and technologies that scientists use to design, perform, analyse and interpret their experiments. The purpose of the 3DbioNet network is to bring together the skills of scientists from many different disciplines within academia and industry needed to identify the stumbling blocks and help building this new way of doing biology.

也许你已经在显微镜下见过细胞了？植物、动物和人类都是由细胞组成的。大约一个世纪以来，科学家们经常在透明的平板(玻璃或塑料)上培养细胞。在平坦的表面上培养细胞非常方便。事实上，这项实验非常成功，以至于我们对细胞核(包含遗传信息)和有丝分裂(提供能量)等细胞成分的许多了解都来自这样的实验；这也包括药物的发现和对其潜在毒性的测试。然而，在真实的生物体中，细胞并不附着在平面上。相反，它们与其三维环境相互作用，其中包括其他细胞以及细胞分泌的因子。科学家们现在越来越意识到在平面上培养细胞和在3D环境中培养细胞之间的这种差异的重要性：它改变了细胞的形状，改变了细胞之间的交流方式，改变了细胞对环境变化的反应。直到最近，动物实验一直是在平面上培养细胞的主要替代方法。虽然动物实验对于我们理解生物学和发现新药仍然是必不可少的，但它们并不能完美地模拟人类生物学，出于伦理原因，减少动物实验的数量也是可取的。在过去的十年里，科学家们已经开始在3D中培养细胞，从而产生微组织，通常是从干细胞开始的。这些由人类来源的细胞制成的微组织与人体组织相似。但是，就像从2D打印到3D打印需要新材料、新软件、新想法和新程序一样，从2D细胞培养到3D细胞培养需要科学家用来设计、执行、分析和解释他们的实验的方法、材料和技术方面的大量创新。3DBioNet网络的目的是汇集学术界和工业界许多不同学科的科学家所需的技能，以确定绊脚石，并帮助建立这种研究生物学的新方式。

项目成果

Tailoring the biofunctionality of collagen biomaterials via tropoelastin incorporation and EDC-crosslinking.

• DOI: 10.1016/j.actbio.2021.08.027
• 发表时间: 2021-08
• 期刊: Acta biomaterialia
• 影响因子: 9.7
• 作者: D. Bax;Malavika Nair;A. Weiss;R. Farndale;S. Best;R. Cameron

Quantification of the nonlinear susceptibility of the hydrogen and deuterium stretch vibration for biomolecules in coherent Raman micro-spectroscopy.

定量氢和氘拉伸振动对相干拉曼微光谱法中生物分子的敏感性。

• DOI: 10.1002/jrs.6164
• 发表时间: 2021-09
• 期刊: JOURNAL OF RAMAN SPECTROSCOPY
• 影响因子: 2.5
• 作者: Boorman, Dale;Pope, Iestyn;Masia, Francesco;Watson, Peter;Borri, Paola;Langbein, Wolfgang
• 通讯作者: Langbein, Wolfgang

An insight into the iPSCs-derived two-dimensional culture and three-dimensional organoid models for neurodegenerative disorders.

• DOI: 10.1098/rsfs.2022.0040
• 发表时间: 2022-10-06
• 期刊: Interface focus
• 影响因子: 4.4

A Systematic Comparative Assessment of the Response of Ovarian Cancer Cells to the Chemotherapeutic Cisplatin in 3D Models of Various Structural and Biochemical Configurations-Does One Model Type Fit All?

• DOI: 10.3390/cancers14051274
• 发表时间: 2022-03-01
• 期刊: Cancers
• 影响因子: 5.2
• 作者: Gupta P;Miller A;Olayanju A;Madhuri TK;Velliou E
• 通讯作者: Velliou E

In vitro and in silico approaches to engineering three-dimensional biological tissues and organoids

• DOI: 10.1098/rsfs.2022.0046
• 发表时间: 2022-08-12
• 期刊: Interface Focus
• 影响因子: 4.4