Modernization of an Integrated Specific Pathogen Free Zebrafish Core Facility

项目摘要 俄勒冈州州立大学辛胡贝尔水生研究实验室（SARL）正在寻求资金， 现代化无特定病原体的斑马鱼综合核心设施。我们的NIH生物医学研究 基地在过去十年中成倍增长，翻新和现代化将确保 它仍然是一个关键的资源，以支持研究增长的完善的NIH中心，个人 NIH支持的研究人员和其他利益相关者合作伙伴。SARL支持俄勒冈州 大学的专题研究领域：可持续地球生态系统科学，健康和保健， 经济繁荣和社会进步。该项目的完成将提供现代， 可持续的、无特定病原体的斑马鱼养殖空间、专业研究空间和高效鱼类 生命支持和畜牧系统。我们的总体目标是：1）改造现有的3，500平方英尺 专门建造的水生设施变成一个高度研究人员访问斑马鱼住房设施，2）取代 过时和小型斑马鱼生命支持系统，3）安装现代斑马鱼住房增强 通过自动化清洁，喂养和远程监控，4）建立专业的斑马鱼室，以支持 短期和长期流通研究，5）增加高容量罐清洗能力，以及6） 提供工程空间，进一步推动技术创新。这次翻新将是一个 现有的研究大楼，拥有10，000平方英尺的翻新空间，可容纳全自动斑马鱼 高通量化学筛选设施，现代成像和分子生物学室，2，300平方英尺 斑马鱼行为研究室、专用隔离室、步入式冷藏室、高- 高速光纤数据传输、大型研究人员办公空间和现代化会议室。一个关键 SARL的设计原则是，它通过提供一个完整的菜单， 服务SARL通过积极推广斑马鱼模型来开展研究项目。 完成这一现代化项目将解决限制斑马鱼进一步增长的瓶颈问题 生物医学研究这项建议是长期承诺的一部分， 先进的水生生物医学研究设施在俄勒冈州州立大学，该地区和国家。

PROJECT SUMMARY The Oregon State University Sinnhuber Aquatic Research Laboratory (SARL) is seeking funds to modernize an integrated specific pathogen-free zebrafish core facility. Our NIH biomedical research base has grown exponentially over the past decade and renovation, and modernization will ensure that it remains a critical resource to support research growth for well-established NIH Centers, individual NIH-supported investigators, and other stakeholder partners. The SARL supports Oregon State University’s thematic research areas: the science of sustainable earth ecosystems, health and wellness, and economic prosperity and social progress. Completion of this project will provide modern, sustainable, specific pathogen-free zebrafish housing space, specialty research space, and efficient fish life support and husbandry systems. Our overarching goals are to 1) Renovate an existing 3,500 ft2 purpose-built aquatic facility into a highly researcher-accessible zebrafish housing facility, 2) Replace obsolete and undersized zebrafish life support system, 3) Install modern zebrafish housing enhanced by automated cleaning, feeding, and remote monitoring, 4) Build specialty zebrafish rooms to support short and long term flow-through studies, 5) Add high capacity tank washing capabilities, and 6) Provide engineering space to further seed technological innovations. This renovation will be part of an existing research building with 10,000 ft2 of renovated space that houses a fully automated zebrafish high throughput chemical screening facility, modern imaging and molecular biology rooms, a 2,300 ft2 room for zebrafish behavioral studies, dedicated quarantine rooms, walk-in cold storage rooms, high- speed fiber-optic data transmission, large researcher office spaces, and modern meeting rooms. A key design principle for the SARL is that it seeds multidisciplinary interactions by providing a full menu of services. The SARL nucleates research programs through aggressive promotion of the zebrafish model. Completing this modernization project will address bottlenecks limiting further growth of zebrafish biomedical research. This proposal is part of the long-term commitment to developing and maintaining advanced aquatic biomedical research facilities at Oregon State University, the region, and nationally.