FUNCTIONAL ANALYSIS OF RNS, A VIRULENCE REGULATOR

毒力调节剂 RNS 的功能分析

• 批准号: 6169411
• 负责人: GEORGE Patrick MUNSON
• 金额: $ 3.92万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6169411
• 关键词: DNA binding protein; Escherichia coli; bacteria infection mechanism; bacterial genetics; enteric bacteria; gene expression; genetic transcription; nuclear runoff assay; pilin; site directed mutagenesis; transcription factor; virulence

DESCRIPTION (Adapted from the applicant's abstract): The expression of virulence factors in many enteric bacterial pathogens is dependent upon a regulatory protein from a family of homologous virulence regulators. Rns is a representative member of this family and is required for the expression of adherent CS1 pili in enterotoxigenic strains of Escherichia coli, a common cause of diarrhea in humans. Rns may regulate the expression of adherent pili in response to environmental conditions that mimic the gastrointestinal tract of the host because CS1 pili are not expressed constitutively and Rns is homologous to the ligand dependent regulator AraC. Alternatively, Rns may be a constitutive activator whose effects are modulated through other regulatory pathways. This study will use in vivo and in vitro analysis of Rns and its DNA binding sites to determine if the activity of Rns is directly or indirectly affected by environmental conditions. The definitive elaboration of the Rns regulatory pathway may present new avenues for disease prevention by illuminating potential therapeutic targets required for the expression of virulence factors in enteric bacterial pathogens.

描述（改编自申请人摘要）： 许多肠道细菌病原体中的毒力因子依赖于 来自同源毒力调节因子家族的调节蛋白。 Rns是 该家族的代表成员，并且需要表达 在大肠杆菌的肠致病性菌株中，粘附的CS1皮利是一种常见的 引起人类腹泻的原因。 Rns可调节粘附分子的表达 响应于模拟胃肠的环境条件的皮利 因为CS1皮利不是组成型表达， 与配体依赖性调节因子AraC同源。 或者，Rns 可以是一种组成型激活剂，其作用通过其它调节剂调节， 调控途径。 本研究将使用体内和体外分析， Rns及其DNA结合位点，以确定Rns的活性是否 直接或间接受环境影响。 The definitive Rns调节途径的详细阐述可能为 通过阐明所需的潜在治疗靶点预防疾病 用于在肠道细菌病原体中表达毒力因子。