CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 6097950
• 负责人: SUNHEE C LEE
• 金额: $ 27.09万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6097950
• 关键词: Alzheimer's disease; aging; biomedical facility; dementia; fluorescence microscopy; human old age (65+); human tissue; immunocytochemistry; neuroanatomy; neuropathology; patient /disease registry; postmortem; stainings; tissue /cell preparation

The purpose of the Neuropathology Core (NP Core) is to perform postmortem protocols on brains of participants in the projects of the Einstein Aging Study (EAS) using state-of-the-art neuropathologic methods. The functions of the NP Core are to collect, bank and distribute postmortem material and data as follows: Integrate activities with the Pathology Departmental on-call service so that CNS autopsies of subjects enrolled in the EAS can be performed in a timely fashion and according to protocol. Perform the neuropathologic evaluations according to protocol and collect standardized neuropathologic data from all brains using the following methods: a. Gross examination, b. Hematoxylin and eosin stained sections, c. Thioflavin-S fluorescent microscopy, and d. PHF-tau immunostained sections for staging Alzheimer type pathology. For cases with vascular lesions, the two neuropathologists independently evaluate gross and microscopic data. A consensus clinicopathological diagnosis is reached. In those cases with either neuronal loses loss or Lewy bodies in the substantia nigra, multiple sections are double immunostained with antibodies to tau and ubiquitin. The density of Lewy bodies as well as the presence and severity of Lewy-types neurites in CA2/3 region of hippocampus are determined. Non-Alzheimer degenerative dementia are characterized with immunocytochemistry and antibodies to neurofilament (SMI-31), ubiquitin and PHF-tau (PHF-1 and TG3). The NP Core provides fixed and frozen brain samples to Project 3 for immunocytochemical, image analytical and enzyme linked immunosorbent assays. Tissue is provided to other investigators from time to time, as requested. The NP Core determines apolipoprotein-E genotypes from brain tissue and on blood samples collected by the Clinical Core. Tissue samples are banked for future studies and neuropathologic studies are performed for any pilot project that may related to the program project. All data is communicated to the Statistical Core, using standardized protocols and procedures.

神经病理核心(NP核心)的目的是进行尸检 爱因斯坦老化计划中参与者的大脑方案 使用最先进的神经病理学方法进行研究(EAS)。这些功能 负责收集、储存和分发尸检材料以及 数据如下： 将活动与病理部门随叫随到的服务整合在一起 登记在EAS中的受试者的中枢神经系统尸检可以在 及时地按照协议行事。 按规程进行神经病理评估并收集 来自所有大脑的标准化神经病理数据，使用以下方法 方法：a.大体检查，b.苏木精-伊红染色切片， C.硫代黄素-S荧光显微镜，d.phf-tau免疫组织化学染色 阿尔茨海默型病理分期切片。 对于有血管病变的病例，两位神经病理学家独立 评估粗略数据和微观数据。临床病理上的共识 诊断结果出来了。 在那些神经元丢失或路易小体丢失的情况下 黑质，多个切片双重免疫组织化学染色 针对tau和泛素的抗体。刘易体的密度以及 丘脑CA2/3区路易型神经突起的存在和严重程度 海马体被测定。 非阿尔茨海默病退行性痴呆的特点是 免疫细胞化学与神经丝抗体(SMI-31)、泛素 和PHF-tau(PHF-1和Tg3)。 NP Core为Project 3提供固定和冷冻的大脑样本 免疫细胞化学、图像分析和酶联免疫吸附剂 化验。组织不时地提供给其他调查人员，如 已请求。 NP核心决定脑组织和其他组织的载脂蛋白-E基因类型 临床中心收集的血液样本。 组织样本被储存起来，用于未来的研究和神经病理学研究 为任何可能与计划相关的试点项目执行 项目。 所有数据都与统计核心通信，使用标准化的 协议和程序。