CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 6267198
• 负责人: SUNHEE C LEE
• 金额: $ 27.19万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6267198
• 关键词: Alzheimer's disease; aging; biomedical facility; dementia; fluorescence microscopy; human old age (65+); human tissue; immunocytochemistry; neuroanatomy; neuropathology; patient /disease registry; postmortem; stainings; tissue /cell preparation

The purpose of the Neuropathology Core (NP Core) is to perform postmortem protocols on brains of participants in the projects of the Einstein Aging Study (EAS) using state-of-the-art neuropathologic methods. The functions of the NP Core are to collect, bank and distribute postmortem material and data as follows: Integrate activities with the Pathology Departmental on-call service so that CNS autopsies of subjects enrolled in the EAS can be performed in a timely fashion and according to protocol. Perform the neuropathologic evaluations according to protocol and collect standardized neuropathologic data from all brains using the following methods: a. Gross examination, b. Hematoxylin and eosin stained sections, c. Thioflavin-S fluorescent microscopy, and d. PHF-tau immunostained sections for staging Alzheimer type pathology. For cases with vascular lesions, the two neuropathologists independently evaluate gross and microscopic data. A consensus clinicopathological diagnosis is reached. In those cases with either neuronal loses loss or Lewy bodies in the substantia nigra, multiple sections are double immunostained with antibodies to tau and ubiquitin. The density of Lewy bodies as well as the presence and severity of Lewy-types neurites in CA2/3 region of hippocampus are determined. Non-Alzheimer degenerative dementia are characterized with immunocytochemistry and antibodies to neurofilament (SMI-31), ubiquitin and PHF-tau (PHF-1 and TG3). The NP Core provides fixed and frozen brain samples to Project 3 for immunocytochemical, image analytical and enzyme linked immunosorbent assays. Tissue is provided to other investigators from time to time, as requested. The NP Core determines apolipoprotein-E genotypes from brain tissue and on blood samples collected by the Clinical Core. Tissue samples are banked for future studies and neuropathologic studies are performed for any pilot project that may related to the program project. All data is communicated to the Statistical Core, using standardized protocols and procedures.

神经病理学核心（NP Core）的目的是进行尸检 爱因斯坦衰老项目参与者大脑协议 使用最先进的神经病理学方法进行研究 (EAS)。功能 NP 核心的职责是收集、储存和分发事后材料 数据如下： 将活动与病理科随叫随到的服务相结合，以便 参加 EAS 的受试者的中枢神经系统尸检可以在 及时时尚并按照协议。 根据协议进行神经病理学评估并收集 使用以下标准对所有大脑的神经病理学数据进行标准化 方法： A．肉眼检查，b．苏木精和伊红染色切片， c.硫磺素-S 荧光显微镜检查，以及 d． PHF-tau 免疫染色 用于对阿尔茨海默病类型病理进行分期的部分。 对于血管病变的病例，两位神经病理学家独立 评估总体和微观数据。临床病理学共识 达到诊断。 在那些神经元丢失或路易体丢失的情况下 黑质，多个切片进行双重免疫染色 tau 和泛素抗体。路易体的密度以及 CA2/3 区域路易型神经突的存在和严重程度 海马体已确定。 非阿尔茨海默病退行性痴呆的特征是 免疫细胞化学和神经丝抗体 (SMI-31)、泛素 和 PHF-tau（PHF-1 和 TG3）。 NP Core 为 Project 3 提供固定和冷冻的大脑样本 免疫细胞化学、图像分析和酶联免疫吸附剂 化验。组织会不时提供给其他研究人员，因为 要求。 NP Core 确定脑组织中的载脂蛋白-E 基因型 临床核心收集的血液样本。 组织样本储存用于未来研究和神经病理学研究 为任何可能与该计划相关的试点项目进行 项目。 所有数据均使用标准化数据传送至统计核心 协议和程序。