COMPUTATIONAL STUDIES OF PRION PROTEIN STRUCTURE

朊病毒蛋白结构的计算研究

• 批准号: 6097930
• 负责人: FRED E COHEN
• 金额: $ 22.21万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6097930
• 关键词: chemical models; computer simulation; conformation; molecular dynamics; nucleic acid repetitive sequence; prions; protein folding; protein isoforms; protein sequence; protein structure; scrapie; site directed mutagenesis; thermodynamics

Prion diseases are diseases of protein conformation where the folding of PrP displays non Anfinsenian behavior. We wish to understand the energy landscape that PrP must confront as it folds to form PrPC under normal physiological conditions and how it can refold to form aberrant pathologic conditions. In this project, we will use lattice models of proteins to explore the general features of sequences that allow prion-like behavior instead of more classical Anfinsenian folding. We will study the relevance of these ideas to the mechanisms for inherited disease. Finally, while we now he a better understanding of the three dimensional structure of PrPC from residues 125-231, we still do not know much about the structure of the holoprotein including the octarepeat region (23-90) complexed to copper. Our insight into the structure of PrPSc is even more limited. However, we believe that we can leverage our results on the PrPSc 106 mini prion to learn more about the structural constraints on PrPSc.

朊病毒病是指朊蛋白的折叠呈现非Anfinsen行为的蛋白质构象疾病。我们希望了解PrP在正常生理条件下折叠形成PrPC时必须面对的能量景观，以及它如何重新折叠形成异常病理条件。在这个项目中，我们将使用蛋白质的晶格模型来探索序列的一般特征，这些序列允许朊病毒样行为，而不是更经典的Anfinsenian折叠。我们将研究这些想法与遗传性疾病机制的相关性。最后，虽然我们现在对PrPC残基125-231的三维结构有了更好的了解，但我们仍然不太了解包括与铜络合的八重复区（23-90）在内的全蛋白的结构。我们对PrPSc结构的了解更加有限。然而，我们相信，我们可以利用我们的结果对PrPSc 106迷你朊病毒了解更多关于PrPSc的结构限制。