DETECTIVE: Dissemination and resistance mechanisms of carbapenem-resistant Gram-negative bacilli

侦探：碳青霉烯类耐药革兰氏阴性杆菌的传播和耐药机制

• 批准号: MR/S013660/1
• 负责人: Alan McNally
• 金额: $ 173.35万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FS013660%2F1
• 关键词: DETECTIVE; Dissemination; resistance; mechanisms; carbapenem

Modern genomic technologies and development of genome analysis tools have been successfully applied to improve our understanding of the emergence, spread, short term evolution, and transmission of clinically important MDR bacteria. Genomic analysis has allowed us to determine with fine scale resolution the role played by inter-hospital patient transfer in national and international dissemination of MDR pathogens. It has allowed us to determine the selective forces which drive the evolution of MDR pathogens in the hospital and within patients and the clinical impact this can impose. Genomic analysis has also been used to identify outbreaks, potential sources of outbreaks, and inform successful interventions to bring an end to outbreaks within hospitals. The scale of the MDR problem in Chinese hospitals is reaching alarming levels. The incidence of morbidity and mortality associated with MDR infections in China has grown year-on-year in the 21st century. Most alarming is the rise in the incidence of infections caused by A. baumannii, E. coli and K. pneumoniae which are resistant to carbapenems, that last line of antibiotics available to treat infections with MDR pathogens. The national CHINET network of clinical microbiology laboratories of 34 sentinel hospitals across China (www.chinets.com) report that in 2017, E. coli, K. pneumoniae and A. baumannii were the top three bacterial species recovered from clinical samples. There were 13,337 known cases of carbapenem resistant A. baumannii (69.3% carbapenem resistance prevalence), 6,434 cases of carbapenem resistant K. pneuomoniae (23% carbapenem resistance prevalence), and 845 cases of carbapenem resistant E. coli (2.3% carbapenem resistance prevalence). It is therefore vital that genomic level investigations of these bacteria are implemented in China immediately, to allow the breakthroughs in understanding transmission, spread, and infection control that have been afforded in the Western world.The proposed research to be conducted by the network will be organised around three independent but inter-connected projects:1) Investigate the reservoirs and routes of transmission of carbapenem resistant K. pneumoniae 2) Investigate the community carriage dynamics of carbapenem resistant E. coli leading to the continuous introduction of strains into hospitals and subsequent infections.3) Investigate the reservoirs and routes of transmission of carbapenem resistant Acinetobacter baumannii.

现代基因组技术和基因组分析工具的发展已成功地应用于提高我们对临床重要MDR细菌的出现、传播、短期进化和传播的理解。基因组分析使我们能够以精细的尺度分辨率确定医院间患者转移在MDR病原体的国内和国际传播中所起的作用。它使我们能够确定在医院和患者体内驱动MDR病原体演变的选择性力量以及这可能产生的临床影响。基因组分析还被用于确定疫情、潜在的疫情来源，并为成功的干预措施提供信息，以结束医院内的疫情。中国医院的MDR问题已经达到了令人担忧的程度。进入世纪，中国MDR感染相关的发病率和死亡率逐年上升。最令人担忧的是A.鲍曼不动杆菌E.埃希菌和这些细菌对碳青霉烯类具有抗性，碳青霉烯类是可用于治疗MDR病原体感染的最后一种抗生素。全国34家哨点医院临床微生物实验室网络（www.chinets.com）报告称，2017年，E. coli，K.肺炎链球菌和A.鲍曼不动杆菌是从临床样品中回收的前三种细菌种类。有13，337例已知的碳青霉烯耐药A。鲍曼不动杆菌对碳青霉烯类耐药率为69.3%;对碳青霉烯类抗生素耐药的大肠埃希菌845例，占23%。大肠埃希菌（2.3%）对碳青霉烯类抗生素耐药。因此，在中国立即开展这些细菌的基因组水平的研究至关重要，以便在了解传播、传播和感染控制方面取得突破，这在西方世界已经提供。该网络将开展的拟议研究将围绕三个独立但相互关联的项目进行组织：1）调查碳青霉烯耐药克雷伯氏菌的储存库和传播途径; 2）调查耐碳青霉烯类大肠杆菌的群落携带动态。3）调查碳青霉烯类耐药鲍曼不动杆菌的宿主和传播途径。

项目成果

Temperature-Regulated IncX3 Plasmid Characteristics and the Role of Plasmid-Encoded H-NS in Thermoregulation.

• DOI: 10.3389/fmicb.2021.765492
• 发表时间: 2021
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Baomo L;Lili S;Moran RA;van Schaik W;Chao Z
• 通讯作者: Chao Z

Global emergence of a hypervirulent carbapenem-resistant Escherichia coli ST410 clone.

• DOI: 10.1038/s41467-023-43854-3
• 发表时间: 2024-01-12
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Ba, Xiaoliang;Guo, Yingyi;Moran, Robert A.;Doughty, Emma L.;Liu, Baomo;Yao, Likang;Li, Jiahui;He, Nanhao;Shen, Siquan;Li, Yang;van Schaik, Willem;McNally, Alan;Holmes, Mark A.;Zhuo, Chao
• 通讯作者: Zhuo, Chao

Prevalence and characterization of mecC MRSA in bovine bulk tank milk in Great Britain, 2017-18.

• DOI: 10.1093/jacamr/dlaa125
• 发表时间: 2021-03
• 期刊: JAC-antimicrobial resistance
• 影响因子: 3.4
• 作者: Cui C;Ba X;Holmes MA
• 通讯作者: Holmes MA

Epidemiology, evolution and cryptic susceptibility of methicillin-resistant Staphylococcus aureus in China: a whole-genome-based survey

• DOI: 10.1016/j.cmi.2021.05.024
• 发表时间: 2021-12-20
• 期刊: CLINICAL MICROBIOLOGY AND INFECTION
• 影响因子: 14.2
• 作者: Chen, Yan;Sun, Lu;Yu, Yunsong
• 通讯作者: Yu, Yunsong

Characterization of phage resistance and phages capable of intestinal decolonization of carbapenem-resistant Klebsiella pneumoniae in mice.

• DOI: 10.1038/s42003-022-03001-y
• 发表时间: 2022-01-13
• 期刊: Communications biology
• 影响因子: 5.9
• 作者: Fang Q;Feng Y;McNally A;Zong Z
• 通讯作者: Zong Z