HYDROGEN PEROXIDE GENERATING LACTOBACILLI AND MONOPEROXIDASE AS MICROBICIDES
HYDROGEN PEROXIDE GENERATING LACTOBACILLI AND MONOPEROXIDASE AS MICROBICIDES
批准号:
6100022
负责人:
SEYMOUR J KLEBANOFF
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-04-01 至 1999-09-29
中文摘要
过氧化物酶,H2O2和卤化物形成一个强大的抗菌系统
英文摘要
Peroxidase, H2O2 and a halide form a powerful antimicrobial system
effective against a variety of microorganisms. Peroxidase is present in
the vaginal fluid or cervical mucous of some, but not all, women. A
primary source of H2O2 in the vagina is lactic acid bacteria including
lactobacilli. However, only half of sexually active women enrolled in
cross-sectional studies have H2O2 producing lactobacilli in the vagina.
Published data has demonstrated that H2O2-producing lactobacilli alone
at high concentrations are viricidal to HlV-1 and at levels where H2O2
producing lactobacilli are ineffective alone, the addition of peroxidase
and halide (Cl-) restored viricidal activity. The long term goals of this
study are to assess the effect of a suppository containing peroxidase
plus H2O2-producing lactobacilli on genital infections.
In this proposed study, the characteristics of the lactobacillus-
peroxidase-halide anti-microbial system will be evaluated in the vagina
of HlV-infectent and noninfected women. A combination suppository
containing both H2O2-generating lactobacilli and recombinant
myeloperoxidase will be developed and tested in vitro for retention of
its bactericidal and viricidal (HlV-1) effectiveness. This combination
suppository will be examined in vivo in nonhuman primates to assess its
effect on the vaginal flora and epithelium. The effect of the suppository
on cervical C. trachomatis and HlV-2 infection in the monkey will be
evaluated. If suitably low toxicity is observed in the monkey model, the
combination suppository will be applied in HlV-infected and uninfected
women to assess whether supplementation of this endogenous microbicide
decreases the ability to detect HlV among women shedding virus. The
effect of the combination suppository on the vaginal flora and epithelium
of HlV-positive and negative women will be evaluated.
This series of studies-will lead to a greater understanding of the role
of the endogenous microbicidal system in the maintenance of a normal
vaginal ecosystem, and will determine whether supplementation with
exogenous lactobacilli and peroxidase could decrease transmission of some
STDs.
This project will involve human subjects recruited through the clinical
core. The suppository will be evaluated in both primates (project 1) and
humans (core D). This project will contribute to the overall program by
better defining the endogenous microbicidal systems in the vagina. This
information has relevance to all aspects of vaginal microbicide
development.
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HYDROGEN PEROXIDE GENERATING LACTOBACILLI AND MONOPEROXIDASE AS MICROBICIDES
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批准号:6235441
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项目类别:
-
资助金额:$9.23万
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财政年份:1997
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负责人:SEYMOUR J KLEBANOFF
-
依托单位:
MAST CELL - EOSINOPHIL INTERACTION IN ALLERGIC INJURY
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批准号:3127432
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项目类别:
-
资助金额:$13.29万
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财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
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依托单位:
MAST CELL--EOSINOPHIL INTERACTION IN ALLERGIC INJURY
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批准号:3127437
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项目类别:
-
资助金额:$19.46万
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财政年份:1981
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负责人:SEYMOUR J KLEBANOFF
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依托单位:
MAST CELL - EOSINOPHIL INTERACTION IN ALLERGIC INJURY
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批准号:3127433
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项目类别:
-
资助金额:$13.35万
-
财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
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依托单位:
MAST CELL--EOSINOPHIL INTERACTION IN ALLERGIC INJURY
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批准号:3127430
-
项目类别:
-
资助金额:$16.72万
-
财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
MAST CELL--EOSINOPHIL INTERACTION IN ALLERGIC INJURY
-
批准号:3127434
-
项目类别:
-
资助金额:$17.53万
-
财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
MAST CELL--EOSINOPHIL INTERACTION IN ALLERGIC INJURY
-
批准号:3127435
-
项目类别:
-
资助金额:$17.83万
-
财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
MAST CELL - EOSINOPHIL INTERACTION IN ALLERGIC INJURY
-
批准号:3127429
-
项目类别:
-
资助金额:$12.14万
-
财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
MAST CELL--EOSINOPHIL INTERACTION IN ALLERGIC INJURY
-
批准号:3127436
-
项目类别:
-
资助金额:$18.73万
-
财政年份:1981
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE - ROLE IN LEUKOCYTES
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批准号:3124386
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项目类别:
-
资助金额:$13.29万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
-
批准号:2058486
-
项目类别:
-
资助金额:$25.27万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
-
批准号:2058485
-
项目类别:
-
资助金额:$24.32万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE--ROLE IN LEUKOCYTES
-
批准号:3480438
-
项目类别:
-
资助金额:$18.76万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
-
批准号:6153984
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项目类别:
-
资助金额:$7.5万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE--ROLE IN LEUKOCYTES
-
批准号:3480436
-
项目类别:
-
资助金额:$18.07万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
-
批准号:3480437
-
项目类别:
-
资助金额:$24.47万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE--ROLE IN LEUKOCYTES
-
批准号:3480440
-
项目类别:
-
资助金额:$20.12万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE - ROLE IN LEUKOCYTES
-
批准号:3124385
-
项目类别:
-
资助金额:$13.67万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
-
批准号:2058487
-
项目类别:
-
资助金额:$26.22万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
-
批准号:2429348
-
项目类别:
-
资助金额:$27.27万
-
财政年份:1978
-
负责人:SEYMOUR J KLEBANOFF
-
依托单位:
海外基金