MAST CELL--EOSINOPHIL INTERACTION IN ALLERGIC INJURY

肥大细胞--过敏性损伤中嗜酸性粒细胞的相互作用

• 批准号: 3127430
• 负责人: SEYMOUR J KLEBANOFF
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3127430
• 关键词: anaphylaxis; basophils; cell cell interaction; chemoattractants; chondroitin sulfates; eosinophil; glycoproteins; guinea pigs; heparin; human subject; laboratory mouse; laboratory rat; leukotrienes; mast cell; neoplasm /cancer immunology; peroxidases; radiotracer; secretion; superoxides; tissue /cell culture

Mast cells and eosinophils are closely associated in allergic reactions. Eosinophil peroxidase (EPO) when combined with hydrogen peroxide (H202) and a halide forms a strong oxidative system capable of initiating mast cell degranulation. EPO also binds firmly to the secreted mast cell granules (MCG) to form a complex with augmented peroxidatic activity. Objectives of this research are 1) to study the formation and biological properties of the MCG-EPO complex; 2) to study the formation and inactivation of leukotrienes by phagocytes; 3) to study the induction of mast cell secretion by agents released by granulocytes; 4) to study the interaction of mast cell and eosinophil products in the killing of the schistosomula of Schistosoma mansoni and 5) to study the degradation of mast cell heparin by granulocyte products. The overall aim is to study the interaction of mast cells and eosinophils and the role of this interaction in the manifestations of allergic diseases and in the host defense against parasites.

肥大细胞和嗜酸性粒细胞在过敏反应中密切相关。 嗜酸性粒细胞过氧化物酶（EPO）与过氧化氢（H2 O2）结合时， 卤化物形成一个强氧化系统， 脱颗粒 EPO还与分泌的肥大细胞颗粒牢固结合 (MCG)以形成具有增强的过氧化物活性的复合物。 目标 本研究的主要目的是：1）研究 MCG-EPO复合物; 2）研究MCG-EPO复合物的形成和失活， 3）研究肥大细胞的诱导作用 通过粒细胞释放的试剂分泌; 4）研究相互作用 肥大细胞和嗜酸性粒细胞产物在杀死 曼氏血吸虫和5）研究肥大细胞肝素降解 粒细胞制品。 总的目的是研究桅杆的相互作用 细胞和嗜酸性粒细胞的作用，这种相互作用在 过敏性疾病的表现和宿主防御 寄生虫