CORE--BIOCHEMICAL SUPPORT

核心--生化支持

• 批准号: 6269171
• 负责人: L L BENNETT
• 金额: $ 29.32万
• 依托单位: SOUTHERN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6269171
• 关键词: biochemistry; biomedical facility; drug screening /evaluation; high performance liquid chromatography; tissue /cell culture

The biochemistry group has a dual role in this program project. It is responsible for the initial biochemical characterization of agents synthesized under this grant, and also performs in-depth biochemical routine determinations and hence appears appropriate for support as a core. Our proposed core consists of (a) a cell culture laboratory that determines cytotoxicities of new agents, collaborates with the biochemists in the performance of routine metabolic experiments in cell culture, and provides cells in quantity for enzyme isolations, and (b) a component for rapidly obtaining a biochemical profile of a new agent by subjecting it to a series of assays including examination by HPLC of its phosphorylation and its effects on nucleotide pools, determinations of its effects on macromolecular syntheses, and determination of its activity as substrate or inhibitor of selected enzymes of nucleoside metabolism.

生物化学组在该计划项目中扮演着双重角色。 这是 负责药剂的初始生化表征 在此资助下合成，并进行了深入的生化研究 常规测定，因此似乎适合作为核心支持。 我们提出的核心包括（a）一个细胞培养实验室，它确定 新药物的细胞毒性，与生物化学家合作 在细胞培养物中进行常规代谢实验，并提供 用于酶分离的大量细胞，以及（b）快速分离的成分 通过一系列的实验来获得新药物的生化特征 包括通过 HPLC 检查其磷酸化及其 对核苷酸库的影响，其影响的测定 大分子合成及其作为底物或活性的测定 核苷代谢选定酶的抑制剂。