CORE--BIOCHEMICAL SUPPORT

生物化学小组在这个项目中扮演着双重角色。它是 负责试剂的初始生物化学表征 在这项资助下合成，并进行深入的生化 这是例行的决定，因此似乎适合作为核心的支助。 我们建议的核心包括(A)一个细胞培养实验室，以确定 新试剂的细胞毒性，与生物化学家在 在细胞培养中进行常规代谢实验，并提供 用于酶分离的细胞数量，以及(B)用于快速分离的成分 通过对新制剂进行一系列试验来获得其生化图谱 检测方法包括用高效液相色谱检测其磷酸化和 对核苷酸池的影响，其影响的测定 大分子合成及其作为底物或底物的活性测定 核苷代谢的特定酶的抑制剂。

The biochemistry group has a dual role in this program project. It is responsible for the initial biochemical characterization of agents synthesized under this grant, and also performs in-depth biochemical routine determinations and hence appears appropriate for support as a core. Our proposed core consists of (a) a cell culture laboratory that determines cytotoxicities of new agents, collaborates with the biochemists in the performance of routine metabolic experiments in cell culture, and provides cells in quantity for enzyme isolations, and (b) a component for rapidly obtaining a biochemical profile of a new agent by subjecting it to a series of assays including examination by HPLC of its phosphorylation and its effects on nucleotide pools, determinations of its effects on macromolecular syntheses, and determination of its activity as substrate or inhibitor of selected enzymes of nucleoside metabolism.