CORE--BIOCHEMICAL SUPPORT

生物化学小组在这个项目中扮演着双重角色。 是 负责病原体的初始生化表征 在这项资助下合成，并进行深入的生化 常规测定，因此似乎适合作为核心支持。 我们建议的核心包括（a）一个细胞培养实验室， 新药剂的细胞毒性，与生物化学家合作， 在细胞培养中进行常规代谢实验，并提供 用于酶分离的大量细胞，和（B）用于快速分离的组分， 通过对新试剂进行一系列生物化学分析， 包括通过HPLC检查其磷酸化及其 对核苷酸库的影响， 大分子合成，并测定其作为底物或 选择核苷代谢酶的抑制剂。

The biochemistry group has a dual role in this program project. It is responsible for the initial biochemical characterization of agents synthesized under this grant, and also performs in-depth biochemical routine determinations and hence appears appropriate for support as a core. Our proposed core consists of (a) a cell culture laboratory that determines cytotoxicities of new agents, collaborates with the biochemists in the performance of routine metabolic experiments in cell culture, and provides cells in quantity for enzyme isolations, and (b) a component for rapidly obtaining a biochemical profile of a new agent by subjecting it to a series of assays including examination by HPLC of its phosphorylation and its effects on nucleotide pools, determinations of its effects on macromolecular syntheses, and determination of its activity as substrate or inhibitor of selected enzymes of nucleoside metabolism.