REGULATION OF THE SYNAPTIC VESICLE CYCLE

突触小泡周期的调节

• 批准号: 6104146
• 负责人: REGIS B KELLY
• 金额: $ 22.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6104146
• 关键词: FK506; HeLa cells; PC12 cells; cell differentiation; cyclic AMP; cyclosporines; dopamine; granule; membrane activity; neuropharmacology; neurotoxins; neurotransmitter transport; neurotrophic factors; phorbols; phosphatidate; protein isoforms; synaptic vesicles; transfection

The synapse is altered by learning and by prolonged exposure to psychoactive drugs.Release of neurotransmitter at the synapse depends on the rates at which synaptic vesicles fuse with the plasma membrane, then recycle and refill. Recently, proteins involved in the fusion step have been identified and shown to form a fusion complex of vesicle and plasma membrane proteins. In addition, an assay has been developed in this lab that screens for the proteins involved in vesicle recycling. The other two proposals also address vesicle refilling. There is evidence that one or more of these three steps in the synaptic cycle are modified by psychoactive drugs. in this proposal assays of fusion complexes and of synaptic vesicle biogenesis will be developed using the neuroendocrine, dopamine-secreting cell line, PC12, since dopamine pathways appear to be central to drug abuse. To determine how the molecules involved in these two steps of the synaptic vesicle cycle are regulated by short-term and long-term modifications, the assays will be used to detect changes in response to secretagogues, NGF, synaptic modulators and selected psychoactive substances.

突触会因学习和长时间暴露于 神经递质在突触处的释放取决于 突触囊泡与质膜融合的速率， 循环再用最近，参与融合步骤的蛋白质已经 被鉴定并显示形成囊泡和血浆的融合复合物 膜蛋白此外，本实验室还开发了一种检测方法， 来筛选参与囊泡循环的蛋白质。另外两 建议还涉及囊泡再填充。有证据表明， 突触周期中的这三个步骤中， 精神药物在该提议中，融合复合物和 突触囊泡生物发生将利用神经内分泌， 多巴胺分泌细胞系，PC12，因为多巴胺途径似乎是 是药物滥用的中心为了确定参与这些的分子是如何 突触囊泡周期的两个步骤由短期和 长期修改后，检测将用于检测 对促分泌素、神经生长因子、突触调节剂和选择性 精神活性物质