Core--Immunohistopathology

核心--免疫组织病理学

• 批准号: 6213507
• 负责人: BRADFORD T STOKES
• 金额: --
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-25 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6213507
• 关键词: autoradiography; biomedical facility; histopathology; immunocytochemistry; immunopathology; in situ hybridization

DESCRIPTION (adapted from investigator's abstract): The Immunohistopathology Core C will provide coordinated and efficient facilities to allow reproducible and predictable histochemical processing of tissue samples that will be subsequently analyzed by automated or semiautomated image analysis techniques. The co-principal investigators will supervise a team that has an established record in quantitative tissue morphometrics, cell counting, immunohistopathology, in situ hybridization, gel analysis, and autoradiography. Accuracy and reproducible outcomes are likely to result from the approach. The co-principal investigators and staff will provide oversight for the facility including scheduling, training of project personnel, and supervising use of the image analysis facilities. The enhanced analysis system that will be used in this proposal is unique in that it replaces a variety of less sensitive, cumbersome technologies that have previously been used for this purpose. Technical personnel in the facility will all be well versed in the employment of the hardware and software in the proposed imaging center, as it applies to immunopathology, densitometry, and fluorescent techniques. The additional technician requested will provide support to Projects 2-4.

描述（根据研究者的摘要进行了改编）：免疫组织病理学核心C将提供协调和有效的设施，以允许对组织样品的可重复可再现和可预测的组织化学处理，随后将通过自动化或半启示图像分析技术对其进行分析。联合主管研究人员将监督一个在定量组织形态计量学，细胞计数，免疫组织病理学，原位杂交，凝胶分析和自显影术方面具有既定记录的团队。准确性和可重复的结果可能是由方法引起的。联合主持的调查人员和工作人员将为该设施提供监督，包括安排，项目人员培训以及监督使用图像分析设施。该提案中将使用的增强分析系统是独一无二的，因为它取代了以前用于此目的的各种敏感，繁琐的技术。该设施中的技术人员将精通拟议的成像中心的硬件和软件的使用，因为它适用于免疫病理学，密度计和荧光技术。要求的其他技术人员将为2-4项目提供支持。