CORE--IMMUNOHISTOPATHOLOGY

核心--免疫组织病理学

• 批准号: 6642243
• 负责人: BRADFORD T STOKES
• 金额: $ 13.78万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642243
• 关键词: automated data processing; autoradiography; biomedical facility; cell sorting; computer data analysis; computer graphics /printing; computer program /software; computer system hardware; data management; densitometry; histopathology; immunocytochemistry; in situ hybridization; light microscopy; personal computers; tissue /cell culture; tissue /cell preparation

The Immunohistopathology Core (Core C) will provide coordinated and efficient facilities to allow reproducible and predictable histochemical processing of tissue samples that will be subsequently analyzed by automated or semi-automated image analysis techniques. Dr. Stokes and Popovich as CO-PI's will supervise a team that has an established record in dealing with the quantitative aspects of tissue morphometrics, cell counting, immunohistopathology, in situ hybridization, gel analysis and autoradiography. Accuracy and reproducible outcomes are therefore likely to result from such an approach. We will provide oversight responsibilities for the facility including scheduling, training of project personnel in histological preparation/analysis and supervise use of the imaging/microscopic facilities as necessary. The enhanced system (Zeiss Axioplan light microscope together with microcomputer imaging software of an MCID 4 system will capacities for darkfield and differential interference contrast (DIC) optics, epi-fluorescence illumination for immunohistochemistry/immunofluorescence and software that facilitates semi-automated cell counts, cell morphometric analysis, and the ability to do fractional densitometric analysis of tissue areas) that will be used in this proposal is unique in that it replaces a variety of less sensitive, cumbersome technologies that have previously been used for this purpose by individual project investigators. Technical personnel in the facility will also be well versed in the employment of the hardware and software proposed in the imaging center, particularly as it applies to immunocytopathology, densitometry, and fluorescent techniques. An additional technician requested in the present proposal will provide support to projects 2-4 involving: (1) methods of fixation, (2) planning and execution of tissue sectioning, (3) appropriate immunohistochemical techniques, and (4) data analysis/management (e.g., stereological techniques, sampling methods, etc.) data storage/cataloging, and data presentation. This technical approach will be aided in data analysis and statistical procedures by a close working relationship between this Core and Core B (Statistical Core). Core B will advise on appropriate database technology or need for convertible modes of data presentation and/or new software for quantitative stereological procedures.

免疫组织病理学核心（核心C）将提供协调和有效的设施，以允许对组织样品的可再现和可预测的组织化学处理，随后将通过自动化或半自动图像分析技术对其进行分析。 Stokes博士和Popovich博士作为Co-Pi's将监督一个在处理组织形态计量学，细胞计数，免疫组织病理学，原位杂交，凝胶分析和自显影的定量方面方面具有既定记录的团队。因此，准确性和可重复的结果可能是由于这种方法而产生的。我们将为设施提供监督责任，包括安排，在组织学准备/分析中对项目人员进行培训，并根据需要监督对成像/显微镜设施的使用。 The enhanced system (Zeiss Axioplan light microscope together with microcomputer imaging software of an MCID 4 system will capacities for darkfield and differential interference contrast (DIC) optics, epi-fluorescence illumination for immunohistochemistry/immunofluorescence and software that facilitates semi-automated cell counts, cell morphometric analysis, and the ability to do fractional densitometric analysis该提案中将使用的组织区域的独特之处在于，它取代了各个项目研究人员以前已将其用于此目的的各种敏感，繁琐的技术。设施中的技术人员也将精通成像中心中提出的硬件和软件的使用，尤其是适用于免疫细胞病理学，密度测定法和荧光技术。本提案中要求的其他技术人员将为2-4的项目提供支持：（1）固定方法，（2）组织切片计划和执行，（3）适当的免疫组织化学技术，以及（4）数据分析/管理（例如，立体技术，样本方法等）数据存储/cataloging/cataloging和Data Presention。这种技术方法将通过该核心和核心B（统计核心）之间的密切工作关系来帮助数据分析和统计程序。核心B将为适当的数据库技术提供建议，或者需要可转换的数据显示模式和/或新软件，以进行定量立体程序。