SPINAL CORD INJURY RESEARCH CENTER

脊髓损伤研究中心

• 批准号: 2262156
• 负责人: BRADFORD T STOKES
• 金额: $ 80.89万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-12-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2262156
• 关键词: nervous system regeneration; spinal cord injury; trauma

Spinal cord injury and its eventual outcome is clearly a by-product of the complex processes of primary and secondary degeneration and the presumptive attempts of the adult spinal cord to express plastic or regenerative behavior after the original lesion. Our Center has used a combination of approaches to examine and manipulate the acute factors that accompany the degenerative phenomenon. In addition, we have exploited the known capacity of young vertebrates to regenerate spinal pathways with our studies that attempt to relate the plastic behavior of fetal spinal pathways to processes of spinal reorganization and development. It is the working hypothesis of our Center that the factors which contribute to a relative lack of spinal regeneration in adult mammals are expressed in such a way that intrinsic growth potentials are never reached. Individual projects span a wide range of disciplines and methodologies in pursuit of these objectives. Our first two projects (1-2) will use our recently developed electro-mechanical impact device to study acute and chronic responses to impact injury, to characterize the neurochemical sequelae to traumatic injury (Project 1) and investigate the physiological roles that fetal transplants may play in recover processes known to be initiated after injury (Project 2). Adult regenerative phenomena and developmental processes related thereto are studied in other projects. Project 3 examines the normal and altered circuitry of specific somatic and visceral reflexes in the cat spinal cord. The delayed development of descending pathways in the opossum is taken advantage of in the next project (Project 4). Since all brainstem pathways seem to have a critical period during which they express plastic behaviors (growing around induced lesions), this project asks questions about the generality of the phenomenon. Ascending systems seem to show similar plasticity in preliminary data presented by this author. This project therefore provides an excellent model system in which a developing mammal can be studied in vivo. Project 5 should allow the elucidation of certain mechanisms of action of gangliosides at the biochemical and molecular level, particularly as they relate to plastic behavior of the central nervous system and growth phenomena related thereto. This project clearly relates to all of those above it since it addresses basic issues related to the ability of gangliosides to modulate biochemical mechanisms of degeneration such as alterations in protein phosphorylation (Project 1), to clarify the role of gangliosides in neuritogenesis during spinal grafting (Project 2) and explore the roles of such substances in developmental plasticity (Projects 3-4). Utilization of such biochemical approaches will undoubtably amplify the anatomical and physiological information to be gathered in similar animals. Such multifaceted approaches have led to important collaborative research that would have otherwise been unlikely outside of the center concept.

脊髓损伤及其最终结果显然是 原发性和次要变性的复杂过程以及 成年脊髓的推定尝试表达塑料或 原始病变后的再生行为。 我们的中心使用了 检查和操纵急性因素的方法的组合 伴随退化现象。 此外，我们还有 利用年轻脊椎动物再生脊柱的已知能力 我们的研究试图将塑料行为与我们的研究相关联 胎儿脊柱通道通往脊柱重组和 发展。 正是我们中心的工作假设是因素 这导致成人相对缺乏脊柱再生 哺乳动物的表达方式是内在生长潜力是 从未达到。 各个项目涵盖了广泛的学科和方法论 追求这些目标。 我们的前两个项目（1-2）将使用 我们最近开发的电力影响装置，以研究急性 和对损伤的慢性反应，以表征神经化学 后遗症到创伤性损伤（项目1）并研究 胎儿移植可能在恢复过程中发挥的生理作用 已知是在受伤后开始的（项目2）。 成人再生 研究了与之相关的现象和发展过程 其他项目。 项目3检查了正常和变化的电路 猫脊髓中的特定体细胞和内脏反射。 这 服用负鼠中下降途径的延迟发展 下一个项目的优势（项目4）。 因为所有的脑干 途径似乎有一个关键时期，在此期间它们表达塑料 行为（围绕诱导病变生长），该项目提出问题 关于现象的普遍性。 上升系统似乎显示 该作者提供的初步数据中的类似可塑性。 这 因此，项目提供了一个出色的模型系统 可以在体内研究发展哺乳动物。 项目5应该允许 阐明了神经节在某些作用机制 生化和分子水平，尤其是与塑料有关的 中枢神经系统和与生长现象相关的行为 那里。 该项目显然与上面的所有内容有关 解决了与神经毒剂调节的能力有关的基本问题 变性的生化机制，例如蛋白质的改变 磷酸化（项目1），以阐明神经节苷脂在 脊柱移植期间的神经发生（项目2）并探索角色 这种物质在发育可塑性中（项目3-4）。 这种生化方法的利用无疑会放大 解剖学和生理信息要聚集 动物。 这种多方面的方法导致了重要的 否则在外面不太可能的协作研究 中心概念。