MASS SPECTROMETRY OF DRUGS, NATURAL PRODUCTS, PROTEINS, AND OLIGONUCLEOTIDES

药物、天然产物、蛋白质和寡核苷酸的质谱分析

• 批准号: 6105233
• 负责人: Lewis K. Pannell
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6105233
• 关键词: aminoacid; antiviral agents; biological products; chemical information system; drug screening /evaluation; electrospray ionization mass spectrometry; marine biology; mass spectrometry; oligonucleotides; peptides; posttranslational modifications; stereoisomer; structural biology

Specialized mass spectrometric analyses are provided to the institute and to other collaborating units. The emphasis is split between the identification of biologically active trace organic compounds isolated from natural sources and the mass spectrometry of biopolymers (e.g. peptide, proteins and oligonucleotides). A very close working relationship and collaboration is maintained with the Laboratory of Biophysical Chemistry, NHLBI. While this group runs many routine analyses for laboratories at NIH, as much emphasis as possible is placed on research using mass spectrometry for the structural elucidation of biologically active compounds. Key projects include: the structural identification of potent anti-HIV and anti-cancer compounds; the identification of post-translational modifications in proteins; continued research on cyanovirin, a potent anti-HIV compound heading towards clinical trial; development of techniques for micro- scale LCMS of biopolymers with the aim of greatly enhancing sensitivity; the identification of Perilipin C which mediates lipid metabolism; an examination of sialic acid in relation to malaria transmission by mosquitos; the identification and chiral determination of abnormal and modified amino acids as found in marine natural products; the identification of modified hemoglobins formed during treatments for sickle cell anaemia; sequence corrections to the hemoglobin alpha- and beta-chains of the rhesus monkey used in sickle cell research; and the development of methods for phospholipid identification. The addition of a new HP-LCMS instrument late last year has drastically expanded the abilities and productivity of the group. A collaboration continues with The National Institute of Standards and Technology (NIST) involving the measurement of mass spectra of organic compounds from NIH, the Walter Reed Army Institute of Research and other government agencies. These are then put into the NIST/EPA/NIH mass spectral database which is in use world wide.

专业的质谱分析是 提供给研究所和其他合作单位。这个 重点是对生物活性的鉴定 从天然来源中分离的痕量有机化合物和 生物聚合物的质谱学(例如，肽、蛋白质和 寡核苷酸)。非常密切的工作关系和 与生物物理实验室保持合作 化学，NHLBI。虽然该小组进行了许多例行分析 对于NIH的实验室，尽可能多地强调 利用质谱学进行结构鉴定的研究 具有生物活性的化合物。重点项目包括：结构 鉴定有效的抗艾滋病毒和抗癌化合物； 蛋白质翻译后修饰的鉴定； 一种有效的抗HIV化合物--氰韦林的继续研究 走向临床试验；微创技术的发展 生物聚合物的规模化LC MS，目的是大大提高 调脂药物Perilipin C的敏感性鉴定 新陈代谢；与疟疾有关的唾液酸检查 蚊子传播；鉴定和手性 中发现的异常和修饰氨基酸的测定 海洋天然产品.改性血红蛋白的鉴定 在治疗镰状细胞性贫血过程中形成的；序列 恒河猴血红蛋白α链和β链的改正 猴子在镰状细胞研究中的应用；以及 磷脂的鉴定方法。添加了一个新的 HP-LCMS仪器在去年年底大幅扩展了 团队的能力和生产力。协作仍在继续 与美国国家标准与技术研究所(NIST) 涉及有机化合物的质谱学测量 来自美国国立卫生研究院、沃尔特里德陆军研究所和其他机构 政府机构。然后将这些放入NIST/EPA/NIH 世界上正在使用的质谱学数据库。