CORE--RNA SYNTHESIS AND RECOMBINANT PROTEIN EXPRESSION FACILITY

RNA合成和重组蛋白核心是一个共享设施 提供试剂，以协助进行拟议的研究; 重组蛋白生产设施将提供试剂。为了 为了促进所提出的实验，毫克量的纯 包含HIV-1基因组的RT-U 5-PBS区域的重组RNA， 需要合成的tRNA。为了提供这项服务，核心将 表达和纯化重组T7 DNA依赖性RNA聚合酶（T7 聚合酶），纯化重组质粒并进行体外转染。 - 是的要求寡核苷酸合成仪生成 特异性修饰的RNA和DNA模板和引物。芯会 提供重组逆转录酶（RT）和核衣壳（NCp 7） proteins.这些活动集中在一个共享资源中 复合物将使这些试剂能够以有效和 成本效益的方式，并将利用现有的实验 这方面的专业知识。

The RNA Synthesis and Recombinant Protein Core is a shared facility which will provide reagents to facilitate the studies proposed; the Recombinant Protein Production Facility will provide reagents. In order to facilitate the experiments proposed, milligram amounts of pure recombinant RNA encompassing the RT-U5-PBS region of HIV-1 genomes and synthetic tRNA will be needed. To provide this service, the Core will express and purify recombinant T7 DNA dependent RNA polymerase (T7 polymerase), purify recombinant plasmids and perform in vitro tran- scriptions. An oligonucleotide synthesizer is requested to generate specifically modified RNAs and DNA templates and primers. The Core will provide recombinant reverse transcriptase (RT) and nucleocapsid (NCp7) proteins. The concentration of these activities in a shared resource complex will enable these reagents to be derived in an efficient and cost effect manner and will capitalize on the available experimental expertise in this area.