CORE--RNA SYNTHESIS AND RECOMBINANT PROTEIN EXPRESSION FACILITY

核心--RNA合成和重组蛋白表达设施

• 批准号: 6204295
• 负责人: STEPHEN L HAJDUK
• 金额: $ 15.97万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6204295
• 关键词: HIV envelope protein; RNA directed DNA polymerase; biomedical facility; molecular cloning; nucleic acid chemical synthesis; recombinant proteins; synthetic nucleic acid

The RNA Synthesis and Recombinant Protein Core is a shared facility which will provide reagents to facilitate the studies proposed; the Recombinant Protein Production Facility will provide reagents. In order to facilitate the experiments proposed, milligram amounts of pure recombinant RNA encompassing the RT-U5-PBS region of HIV-1 genomes and synthetic tRNA will be needed. To provide this service, the Core will express and purify recombinant T7 DNA dependent RNA polymerase (T7 polymerase), purify recombinant plasmids and perform in vitro tran- scriptions. An oligonucleotide synthesizer is requested to generate specifically modified RNAs and DNA templates and primers. The Core will provide recombinant reverse transcriptase (RT) and nucleocapsid (NCp7) proteins. The concentration of these activities in a shared resource complex will enable these reagents to be derived in an efficient and cost effect manner and will capitalize on the available experimental expertise in this area.

RNA合成和重组蛋白核心是一个共享的设施