CORE--RNA SYNTHESIS AND RECOMBINANT PROTEIN EXPRESSION FACILITY
CORE--RNA SYNTHESIS AND RECOMBINANT PROTEIN EXPRESSION FACILITY
批准号:
6204295
负责人:
STEPHEN L HAJDUK
金额:
$15.97万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-09-01 至 2000-08-31
中文摘要
RNA合成和重组蛋白核心是一个共享的设施
将提供试剂以促进拟议的研究;
重组蛋白生产设施将提供试剂。按顺序
为了方便实验建议,毫克量的纯
包含HIV-1基因组RT-U5-PBS区域和
将需要合成的tRNA。为了提供这项服务,核心将
重组T7依赖DNA的RNA聚合酶(T7)的表达和纯化
聚合酶),纯化重组质粒,并进行体外转基因
剧本。一种寡核苷酸合成器被要求生成
经过特别修饰的RNA、DNA模板和引物。核心意志
提供重组逆转录酶(RT)和核衣壳(NCp7)
蛋白质。将这些活动集中在共享资源中
复合体将使这些试剂能够以高效和
成本效益的方式,并将利用现有的试验
这方面的专业知识。
英文摘要
The RNA Synthesis and Recombinant Protein Core is a shared facility
which will provide reagents to facilitate the studies proposed; the
Recombinant Protein Production Facility will provide reagents. In order
to facilitate the experiments proposed, milligram amounts of pure
recombinant RNA encompassing the RT-U5-PBS region of HIV-1 genomes and
synthetic tRNA will be needed. To provide this service, the Core will
express and purify recombinant T7 DNA dependent RNA polymerase (T7
polymerase), purify recombinant plasmids and perform in vitro tran-
scriptions. An oligonucleotide synthesizer is requested to generate
specifically modified RNAs and DNA templates and primers. The Core will
provide recombinant reverse transcriptase (RT) and nucleocapsid (NCp7)
proteins. The concentration of these activities in a shared resource
complex will enable these reagents to be derived in an efficient and
cost effect manner and will capitalize on the available experimental
expertise in this area.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Role of African Trypanosome Extracellular Vesicles in Infection and Pathogenesis
-
批准号:9311314
-
项目类别:
-
资助金额:$37.5万
-
财政年份:2017
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Role of African Trypanosome Extracellular Vesicles in Infection and Pathogenesis
-
批准号:9418021
-
项目类别:
-
资助金额:$37.5万
-
财政年份:2017
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Role of African Trypanosome Extracellular Vesicles in Infection and Pathogenesis
-
批准号:10088373
-
项目类别:
-
资助金额:$37.5万
-
财政年份:2017
-
负责人:STEPHEN L HAJDUK
-
依托单位:
2014 Biology of Host-Parasite Interactions Gordon Research Conference
-
批准号:8716948
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项目类别:
-
资助金额:$0.6万
-
财政年份:2014
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Function of mRNA Editing in Trypanosomes
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批准号:7880344
-
项目类别:
-
资助金额:$3.45万
-
财政年份:2009
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Mechanism of tRNA Import Into Trypanosome Mitochondria
-
批准号:7383137
-
项目类别:
-
资助金额:$34.3万
-
财政年份:2005
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Mechanism of tRNA Import Into Trypanosome Mitochondria
-
批准号:6919501
-
项目类别:
-
资助金额:$38.69万
-
财政年份:2005
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Mechanism of tRNA Import Into Trypanosome Mitochondria
-
批准号:7609075
-
项目类别:
-
资助金额:$34.3万
-
财政年份:2005
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Mechanism of tRNA Import Into Trypanosome Mitochondria
-
批准号:7188655
-
项目类别:
-
资助金额:$34.96万
-
财政年份:2005
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Mechanism of tRNA Import Into Trypanosome Mitochondria
-
批准号:7023071
-
项目类别:
-
资助金额:$9.92万
-
财政年份:2005
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Mechanism of tRNA Import Into Trypanosome Mitochondria
-
批准号:7328313
-
项目类别:
-
资助金额:$27.79万
-
财政年份:2005
-
负责人:STEPHEN L HAJDUK
-
依托单位:
Lysis of Trypanosomes by Haptoglobin Related Protein
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批准号:6333187
-
项目类别:
-
资助金额:$28.7万
-
财政年份:2001
-
负责人:STEPHEN L HAJDUK
-
依托单位:
CORE--RNA SYNTHESIS AND RECOMBINANT PROTEIN EXPRESSION FACILITY
-
批准号:6354727
-
项目类别:
-
资助金额:$15.97万
-
财政年份:2000
-
负责人:STEPHEN L HAJDUK
-
依托单位:
BIRMINGHAM SCIENCE EDUCATION PARTNERSHIP (BSEP) PHASE I
-
批准号:6529914
-
项目类别:
-
资助金额:$32.4万
-
财政年份:2000
-
负责人:STEPHEN L HAJDUK
-
依托单位:
BIRMINGHAM SCIENCE EDUCATION PARTNERSHIP (BSEP) PHASE I
-
批准号:6287129
-
项目类别:
-
资助金额:$31.74万
-
财政年份:2000
-
负责人:STEPHEN L HAJDUK
-
依托单位:
IN VITRO STRUCTURAL ANALYSIS OF THE HIV 1 INITIATION COMPLEX
-
批准号:6354724
-
项目类别:
-
资助金额:$15.97万
-
财政年份:2000
-
负责人:STEPHEN L HAJDUK
-
依托单位:
BIRMINGHAM SCIENCE EDUCATION PARTNERSHIP (BSEP) PHASE I
-
批准号:6394841
-
项目类别:
-
资助金额:$31.19万
-
财政年份:2000
-
负责人:STEPHEN L HAJDUK
-
依托单位:
IN VITRO STRUCTURAL ANALYSIS OF THE HIV 1 INITIATION COMPLEX
-
批准号:6204292
-
项目类别:
-
资助金额:$15.97万
-
财政年份:1999
-
负责人:STEPHEN L HAJDUK
-
依托单位:
CORE--RNA SYNTHESIS AND RECOMBINANT PROTEIN EXPRESSION FACILITY
-
批准号:6107817
-
项目类别:
-
资助金额:$15.97万
-
财政年份:1998
-
负责人:STEPHEN L HAJDUK
-
依托单位:
IN VITRO STRUCTURAL ANALYSIS OF THE HIV 1 INITIATION COMPLEX
-
批准号:6240684
-
项目类别:
-
资助金额:$17.9万
-
财政年份:1997
-
负责人:STEPHEN L HAJDUK
-
依托单位:
海外基金