NEW VARIABLE SEQUENCES

新的变量序列

• 批准号: 6107486
• 负责人: LUIGI L CAVALLI-SFORZA
• 金额: $ 9.39万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6107486
• 关键词: Chinese American; caucasian American; genetic polymorphism; human genetic material tag; human tissue; male; nucleic acid repetitive sequence; sequence tagged sites; sex chromosomes

The general aim of this subproject is the discovery and analysis of variable sequences by new methods. The initial search will be made on a panel of DNA's from individuals chosen to represent the world variation. Major methods and strategies include: 1) Unique sequences, originating from sequence tagged sites (STS's) already available for the Y chromosomes, and new ones to be obtained from Y chromosome YACs will be screened for sequence variation by direct sequencing, following the method summarized in the progress report and the research design below. 2) A search of highly variable sequences will be set up using new mismatch repair methods to be developed. They will be developed for all chromosomes, and also for the Y and X chromosome. 3) We plan to increase our collection of data on mitochondrial DNA variation of the 2 segments of the D-loop, on populations of the circum- mediterranean Basin, including Caucasoids and mixed populations of North and East Africa, and Chinese ethnic populations. 4) We plan to continue obtaining, extending and analyzing results with microsatellites, with a variety of new methods. Problems of evolutionary importance based on mutation rates, natural selection, optimal genetic distances will be paid special attention. We are also interested in carefully testing methods lending themselves for use in less well equipped laboratories which do not have access to radioisotopes or expensive equipment and reagents, so that studies of Human Genome Diversity can be carried out in all countries of the world and contribute efficiently to technology transfer to the South (the Third World).

这个子项目的总体目标是发现和分析 用新的方法得到可变序列。最初的搜索将在一个 来自被选中代表世界变化的个体的DNA小组。 主要方法和战略包括： 1)唯一序列，源自序列标记位点(STS) 已经可以用于Y染色体，新的染色体将从 Y染色体YAC的序列变异将通过直接 按照进度报告中总结的方法进行排序 研究设计如下。 2)搜索高度可变的序列将使用新的 待开发的失配修复方法。它们将为所有人开发 染色体，还有Y和X染色体。 3)我们计划增加对线粒体DNA数据的收集 D-环的两个节段在环状种群中的变异 地中海盆地，包括高加索人和北部的混合人口 和东非，以及华裔人口。 4)我们计划继续获取、扩展和分析结果， 微卫星，具有多种新方法。进化论问题 基于突变率、自然选择、最佳遗传的重要性 距离将被特别注意。我们还感兴趣的还有 仔细测试用于装备较差的设备的方法 无法获得放射性同位素或价格昂贵的实验室 设备和试剂，以便对人类基因组多样性的研究 在世界各国实施，并有效地促进 向南方(第三世界)转让技术。