CORE FACILITIES

核心设施

• 批准号: 6271736
• 负责人: LUIGI L CAVALLI-SFORZA
• 金额: $ 9.06万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271736
• 关键词: biomedical facility; cell bank /registry; computer assisted sequence analysis; molecular cloning; nucleic acid sequence

The core serves purposes of general utility and will continue immortalizing cell lines by EB virus transformation from blood samples received from other projects; when necessary, it will provide cytogenetic localization of probes by somatic cell analysis and by "in situ" hybridizations. The computer work related to linkage data will be carried out in the CORE; and new computer programs, the testing by computer simulation of new methods and the statistical support to investigators of the program project, will be mostly done in this group. The new automated instruments now being developed will be operated and maintained by the CORE. Among these is foreseen the acquisition of an automatic thermocycling reactor for PCR (polymerase chain reaction). There is also partial access to an automatic DNA extractor.

核心服务的目的，一般效用，并将继续 EB病毒血转化永生化细胞系 从其他项目收到的样品;必要时， 通过体细胞分析提供探针的细胞遗传学定位 和“原位”杂交。 计算机相关工作 连接数据将在CORE中进行;和新的计算机 程序，通过计算机模拟新方法的测试， 对项目研究人员的统计支持，将 大部分都是在这个群体中完成的。 新的自动化仪器现在 将由CORE运行和维护。 之间 这些都是可以预见的自动热循环的收购 PCR反应器（聚合酶链式反应）。 也有部分 自动DNA提取器