Cell-Cell Communication Analysis of Cancer Organoids Using Multimodal Single-Cell PTM and RNA Profiling

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Studying how different cell-types collaborate is essential to our understanding of healthy tissues and diseases such as cancer. To investigate how tissues function, we need to measure both how signals are transferred between cells and how that information is processed within cells. Unfortunately, no technology currently exists to simultaneously measure signalling both within and between cells. We have previously developed a powerful technology to measure lots of signals within individual cell-types. Each of these signalling events describe how the cells are behaving - so being able to measure lots of them across different cells is extremely exciting. Unfortunately, our technology cannot measure communication signals between different cells. It has recently been shown that DNA-sequencing technologies can be adapted to study communication between cells. Unfortunately, these sequencing technologies cannot measure signals inside cells. The aim of this project is to develop a technology capable of measuring signalling both within and between single-cells in cancer. By integrating our existing single-cell signalling methods with new single-cell sequencing approaches, we will develop a 'multimodal' technology to study how different cell-types communicate in 'mini-organ' models of bowel cancer. Through understanding how different cells types collaborate to drive tumours, this work will provide novel therapeutic opportunities to treat bowel cancer.