12 DETECTORS 96 WELL MICROPLATE SCINT--LUMINES COUNTER

12 个探测器 96 孔微孔板 SCINT--发光计数器

• 批准号: 2503792
• 负责人: JAMES E HILDRETH
• 金额: $ 8.93万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503792
• 关键词: biomedical equipment; biomedical equipment purchase; luminescence; scintillation counter

Funds are requested for a TopCount bench top microplate scintillation and luminescence counter system, configured for direct scintillation and luminescence counting of samples contained in 96-well microplates. The system will count any isotope that can be used with conventional liquid scintillation counting, as both liquid and solid scintillation counting. In addition the system will accommodate microplates from a number of different manufacturers that are designed for filter and membrane counting, adherent cell cultures, inplate immunobinding, and scintillation proximity assays. The system also includes a 96-well harvester to transfer samples from liquid suspensions onto filters for several of the above applications. Collectively, the system saves a great deal of time by eliminating the need for individual sample handling for washing samples, transfer to vials for radioactive counting, and disposal. Experience has shown that the time saved per 96-well plate is approximately 10 hours. With the use of hundreds of plates per year, this represents a remarkable increase in research efficiency. In addition to the great saving in time, the system will also greatly reduce the exposure of laboratory personnel to repetitive handling of potentially biohazardous materials as occurs with individual sample handling and produces less radioactive waste. This instrument will be housed in the Johns Hopkins University basis science core instrument facility, which has over eleven years of experience and is used by faculty throughout this institution. The initial primary users group are scientists whose research is directed to the study of viral infections that play a major role in human diseases, including human immunodeficiency virus, human papilloma virus and cervical cancer, and cytomegalovirus. It is expected that with the versatility of the instrument ant its availability in the core laboratory, the use will expand to a much greater number of investigators working with adhesion molecules, antibodies, DNA, RNA, proteins, and other experimental systems that can be adapted to the 96-well format instead of single tubes.

请求提供资金，用于TopCount台式微量板闪烁 和发光计数器系统，配置用于直接闪烁， 对包含在96孔微量培养板中的样品进行发光计数。 的 该系统将对可与常规液体一起使用的任何同位素进行计数 闪烁计数，如液体和固体闪烁计数。 此外，该系统将容纳来自多个 为过滤器和膜设计的不同制造商 计数、贴壁细胞培养、板内免疫结合和 闪烁接近测定。 该系统还包括一个96孔 收集器，用于将样品从液体悬浮液转移到过滤器上， 以上几种应用。 总的来说，系统保存了 通过消除对单个样品的需要， 处理清洗样品，转移至小瓶中进行放射性分析 清点和处理 经验表明， 96-孔板约10小时。使用数百个 这代表了研究的显著增长， 效率 除了节省大量时间外，该系统还将 也大大减少了实验室人员对重复性 处理潜在的生物危害材料，如个人 样品处理和产生更少的放射性废物。 本文书 将被安置在约翰霍普金斯大学基础科学中心 仪器设施，拥有超过十一年的经验， 供全校教职员工使用初始用户 这些科学家的研究方向是研究病毒 在人类疾病中起主要作用的感染， 免疫缺陷病毒、人乳头瘤病毒和宫颈癌，以及 巨细胞病毒 可以预期，随着该系统的多功能性， 仪器及其在核心实验室的可用性，使用将 扩大到更多的研究人员与粘附 分子、抗体、DNA、RNA、蛋白质和其他实验性 系统可以适应96孔格式，而不是单一的 管.