PHOTOCHEMISTRY AND PHOTOBIOLOGY OF MATRIX COLLAGENS

阳光暴晒明确地与表皮(前)恶性肿瘤有关 和皮肤的“光老化”。紫外光与紫外光的直接作用 细胞外基质胶原蛋白可能起重要作用。活体真皮 紫外线对胶原酶的刺激导致胶原损伤 真皮成纤维细胞的活性，这可能是部分结果 照片转化的(外来的)胶原蛋白。同样，照片变换 血管和/或基底胶原可能会提供一个不利的环境 对于内皮细胞(EC)的生长和活力，导致改变 细胞骨架形态，以及其他因素对观察到的总数的影响 紫外线对这些组件造成的损害。为了解决这一假设，我们 建议研究不同波长对结构和结构的影响 真皮和基质胶原蛋白以及重组基底膜的功能 (MATRIGEL)膜，并确定这些光转化 可能会引起胶原酶的产生和/或EC的改变。我们将(1)照射 纯化的胶原蛋白具有不同波长的紫外线。我们将监控变化 在结构，比浊度，氨基酸图谱，易感性 胶原酶，荧光性质。(2)培养真皮成纤维细胞， EC使用(照片转化的)胶原蛋白。在(未)辐照材料上生长EC 或将其与III型或IV型胶原蛋白孵育。成纤维细胞会 在正常情况下进行活性、生长和胶原酶诱导的测试 和UV变换的矩阵。EC将接受生存能力、增长能力、 移动性和细胞骨架形态的变化。(3)评估保护措施 标准防晒制剂对体外胶原蛋白的抑制作用 由太阳波长造成的损害。这些研究的结果应该会让人有洞察力 光致癌、光老化和创伤中的重要过程 治愈，可用于化妆品和防晒。 工业。

Sun exposure is unequivocally associated with epidermal (pre)malignancies and with dermal "photo-aging". The direct interaction between UV and extracellular matrix collagens may play an important role. In vivo dermal collagen damage results from UV-induced stimulation of collagenase activity in dermal fibroblasts, which could arise partly as a consequence of photo-transformed ("foreign") collagen. Similarly, photo-transformation of vascular and/or basement collagens may provide a milieu unfavorable for endothelial cell (EC) growth and viability, result in altered cytoskeletal morphology, and otherwise contribute to the total observed UV-induced damage to these components. To address this hypothesis, we propose to study the effect of various wavelengths on the structure and function of dermal and matrix collagens as well as reconstituted basement (MATRIGEL) membranes and to ascertain whether these photo-transformations may elicit collagenase production and/or EC changes. We will (1) Irradiate purified collagens with various wavelengths of UV. We will monitor changes in structure, turbidimetry, amino acid profile, susceptibility to collagenase, fluorescence properties. (2) Incubate dermal fibroblasts and EC with (photo-transformed) collagens. Grow EC on (un)irradiated MATRIGEL matrix or incubate them with types III or IV collagens. Fibroblasts will be tested for viability, growth, and for collagenase induction on normal and UV-transformed matrices. Ec will be tested for viability, growth, mobility, and cytoskeletal morphology changes. (3) Assess the protective effect for standard sunscreen preparations against in vitro collagen damage by solar wavelength. Results from these studies should shed insight into processes important in photo-carcinogenesis, photo-aging and wound healing, and could be applicable to the cosmetic and photo-protection industries.