DEPARTMENTAL MASS SPECTROMETRY FACILITY

部门质谱设施

• 批准号: 2503029
• 负责人: TIMOTHY L MACDONALD
• 金额: $ 22.02万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-15 至 1999-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503029
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; mass spectrometry

This application seeks funds for the purchase of two mass spectrometers to comprise a departmental facility: (1) a Finnegan LCQ Benchtop MS System with electrospray and chemical ionization capability, associated gradient LC pump system, positive- and negative-ion detection, mass/charge range of 50-2000 daltons; and (2) a Finnegan MAT Model SSQ 7000 MS/DS mass spectrometer with electron impact, chemical ionization, and fast atom bombardment capability, positive- and negative-ion detection, and mass/charge range to 2500 daltons. Both systems include powerful and versatile bundled computer systems for instrument control and data manipulation. The proposed equipment is designed to replace the single outdated departmental instrument, which can no longer be brought to adequate levels of performance. The acquisition of reliable mass spectral data is vital to the activities of all of the research groups in the department that engage in chemical and biochemical synthesis. The current lack of this capability is a major impediment to the progress of a variety of NIH- funded programs as well as projects supported by other agencies. The requested instruments are modern "workhorse" machines that have important capabilities previously unavailable to departmental users. The instruments are designed to accomodate the two major types of samples generated by our research groups: standard solid and liquid small-molecule samples, including organic, inorganic, and organometallic compounds, will be served by the SSQ-7000, whereas biological molecules, biopolymers and other large and small polarizable structures will be routinely analyzed with the LCQ electrospray instrumentation. Given the vary active nature of the department's synthetic efforts concerning both types of molecules, the user base for the two instruments will be large.

本申请寻求资金购买两台质谱仪 组成部门设施：(1) Finnegan LCQ Benchtop MS 系统 具有电喷雾和化学电离能力，相关梯度 LC 泵系统、正离子和负离子检测、质量/电荷范围 50-2000道尔顿； (2) Finnegan MAT 型号 SSQ 7000 MS/DS 质量 具有电子轰击、化学电离和快原子功能的光谱仪 轰击能力、正离子和负离子检测，以及 质量/电荷范围可达 2500 道尔顿。 这两个系统都包括强大的和 用于仪器控制和数据的多功能捆绑计算机系统 操纵。 拟议的设备旨在取代单一过时的 部门工具已无法达到足够的水平 的性能。 获取可靠的质谱数据至关重要 该部门所有研究小组的活动 从事化学和生化合成。 目前缺乏这个 能力是各种 NIH 进步的主要障碍 资助的计划以及其他机构支持的项目。 这 所要求的仪器是现代“主力”机器，具有重要的 部门用户以前无法使用的功能。 这 仪器设计用于容纳两种主要类型的样品 由我们的研究小组生成：标准固体和液体小分子 样品，包括有机、无机和有机金属化合物， 由 SSQ-7000 提供服务，而生物分子、生物聚合物和 其他大大小小的可极化结构将被常规分析 使用 LCQ 电喷雾仪器。 鉴于不同的活跃性质 该部门对两种类型分子的合成工作， 这两种工具的用户基础将会很大。