Enhanced sensitivity of electrospray ionization mass spectrometry

提高电喷雾电离质谱的灵敏度

• 批准号: LP160100657
• 负责人: Prof Heike Ebendorff-Heidepriem
• 金额: $ 34.76万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-12 至 2021-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP160100657
• 关键词: Enhanced; sensitivity; electrospray; ionization; mass

Enhanced sensitivity of electrospray ionization mass spectrometry. This project aims to enhance the sensitivity of nano-electrospray ionization mass spectrometry (nanoESI-MS) by an order of magnitude by simultaneously overcoming the two interdependent limitations in ion generation and transmission efficiency. This project will design glass capillaries and tubes with complex structures to enable both multiplexing ion generation from a single capillary and geometrically matching the bore of the tube collecting the emitted ion plume. NanoESI-MS has become an indispensable analytical tool for proteomics and synthetic chemistry. The significant enhancement of nanoESI-MS sensitivity in this project is expected to accelerate progress in disease research, biomarker discovery and drug development.

提高电喷雾电离质谱法的灵敏度。本项目旨在通过同时克服离子产生和传输效率两个相互依赖的限制，将纳米电喷雾电离质谱（nanoESI-MS）的灵敏度提高一个数量级。该项目将设计具有复杂结构的玻璃毛细管和管，以实现从单个毛细管产生多路离子，并在几何上匹配收集发射离子羽流的管的孔。纳米esi - ms已成为蛋白质组学和合成化学不可缺少的分析工具。该项目对纳米esi - ms灵敏度的显著提高有望加速疾病研究、生物标志物发现和药物开发的进展。