PURCHASE OF AN X RAY GENERATOR AND IMAGE PLATE DETECTOR

购买 X 射线发生器和图像板探测器

• 批准号: 2472074
• 负责人: Ronen Marmorstein
• 金额: $ 26.64万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 1999-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2472074
• 关键词: X ray crystallography; biomedical equipment purchase; structural biology

Funds are requested to purchase a Rigaku RU-300 X-ray generator, R-AXIS IV image plate system and X-stream cryogenic crystal freezing apparatus, and a Silicon Graphics computer work station to facilitate X-ray crystallographic studies of macromolecules. The existing instrumentation has facilitated a major expansion of crystallographic investigations at the Wistar Institute. This increased activity in structural studies has reached the point where the lack of sufficient instrumentation resources is significantly impeding progress in studying the structures of important macromolecules. The requested instrumentation will provide the resources required to meet the escalating crystallographic demands of the Institute to a major user group composed of 8 laboratories with substantial NIH grant support. These laboratories (Berger, Burnett, Halazonetis, Herlyn, Lieberman, Marmorstein Rauscher, and Speicher) are studying the structure and function of a wide range of macromolecules from nuclear transcription factors and cytoskeletal proteins to whole viruses. The X-ray instrumentation will be housed in the X-ray diffraction laboratory at The Wistar Institute. The instrumentation will be jointly managed by the P.I. (Dr. Ronen Marmorstein) and the Co-P.I. (Dr. Roger M. Burnett). They will be responsible for instrument installation, training, and the day-to-day operational. An internal advisory committee comprised of all major users, and the Associate Director of Science Administration, will oversee equitable access, operation and administration of the instrument. The Wistar Institute is committed to housing, maintaining and staffing the requested instrument for its useful lifetime as verified by an accompanying letter from the Director.

申请资金用于购买理学RU-300 X射线发生器R-AXIS IV成像板系统和X流低温晶体冷冻装置， 和一个硅图形计算机工作站， 大分子的晶体学研究。现有仪器 促进了晶体学研究的重大扩展， Wistar研究所结构研究活动的增加， 达到了缺乏足够的仪器资源的地步， 严重阻碍了研究重要的 大分子请求的检测将提供资源 以满足研究所不断升级的晶体学需求 由8个实验室组成的主要用户组， 给予支持。这些实验室（伯杰，伯内特，Halazonetis，赫林， Lieberman，Marmorstein Rauscher，and Speicher）正在研究这种结构 和功能的一个广泛的大分子从核转录 因子和细胞骨架蛋白到整个病毒。 X射线仪器将被安置在X射线衍射 Wistar研究所的实验室。仪器将联合 由私家侦探管理(Dr. Ronen Marmorstein）和Co-P.I. (Dr.罗杰·M Burnett）。他们将负责仪器安装，培训， 和日常运作 一个内部咨询委员会， 所有主要用户和科学管理副主任， 将监督公平进入、运作和管理 仪器Wistar研究所致力于提供住房、维护和 在所要求的仪器的使用寿命内配备人员， 一封来自主任的信。