LCQ MASS SPECTROMETER SYSTEM

LCQ 质谱仪系统

• 批准号: 2503103
• 负责人: MICHAEL BALAZY
• 金额: $ 9.35万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 1999-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503103
• 关键词: arachidonate; biomedical equipment; biomedical equipment purchase; eicosanoid metabolism; electrospray ionization mass spectrometry; structural biology

This proposal seeks funds to purchase an LCQ TM MS n ion trap mass spectrometer system (Finnigan Corp., San Jose, CA) for the Biomedical Mass Spectrometry Core Laboratory located in the Department of Pharmacology of New York Medical College, Valhalla, NY. The new instrument will be utilized in the electrospray ionization (ESI/MS) mode and in the electrospray tandem mass spectrometry mode (ESI/MS/MS) to elucidate structures of new complex biomolecules. The new instrument will be used primarily for analyses of arachidonic acid (AA) metabolites which cannot be analyzed using our existing GC/MS instrument (HP 5989A, Mass Engine). These include phospholipids containing esterified hydroxy-AA (HETE), glutathione conjugates of epoxy-AA (EET), diglycerides containing HETE, glucuronide of 20-HETE, and nucleic acid epoxyallylic alcohol adducts. These compounds in an intact form are not suitable for GC/MS analyses primarily because of high polarity and thermal instability. We also plan to develop methods to analyze, quantitatively and simultaneously, several AA metabolites in one injection. This will allow for a more comprehensive look into metabolism of AA by cytochrome P450 and lipoxygenases. In one project, we propose to study the chemistry of the reaction of glutathione with peroxynitrite which requires analysis of nitro- and nitrosoglutathione. We obtained preliminary data which suggest that these compounds are well analyzed by ESI/MS/MS. The proposed analyses focus on the generation of the new compounds in the vascular system, in the kidney, and in tumor cells and the new data is expected to provide significant advances in the areas of vascular biology, renal function and cancer. The major user group consists of six scientists funded by the NIH (3 MERIT awardees and 2 Program Project Grant Investigators) who have projects addressing various areas of pharmacology and biochemistry of vascular system, kidney and cancer. The long term goals are to provide ESI/MS(/MS) analyses for long-term funded projects as well as for new projects. The new instrumentation will lead to major advances in understanding the role of eicosanoids, and glutathione in health and disease.

该提案寻求资金购买 LCQ TM MS n 离子阱质量 用于生物医学的光谱仪系统（Finnigan Corp.，圣何塞，加利福尼亚州） 质谱核心实验室位于该系 纽约医学院药理学，纽约州瓦尔哈拉。 新的 仪器将在电喷雾电离 (ESI/MS) 模式下使用 并在电喷雾串联质谱模式 (ESI/MS/MS) 中 阐明新的复杂生物分子的结构。 新仪器 主要用于花生四烯酸 (AA) 代谢物的分析 无法使用我们现有的 GC/MS 仪器（HP 5989A、 质量引擎）。 这些包括含有酯化的磷脂 羟基-AA (HETE)、环氧-AA (EET) 谷胱甘肽缀合物、 含有HETE的甘油二酯、20-HETE的葡萄糖苷酸和核酸 环氧烯丙醇加合物。 这些完整形式的化合物不 适合 GC/MS 分析，主要是因为高极性和 热不稳定。 我们还计划开发方法来分析， 定量地、同时地，将多种 AA 代谢物集中在一个 注射。 这将有助于更全面地了解 AA 通过细胞色素 P450 和脂氧合酶代谢。 在一个项目中， 我们建议研究谷胱甘肽与 过氧亚硝酸盐，需要分析硝基谷胱甘肽和亚硝基谷胱甘肽。 我们获得的初步数据表明这些化合物很好 通过 ESI/MS/MS 进行分析。 拟议的分析重点关注发电 血管系统、肾脏和肿瘤中的新化合物 细胞和新数据预计将在 血管生物学、肾功能和癌症领域。 主要 用户组由 NIH 资助的 6 名科学家组成（3 MERIT 拥有项目的获奖者和 2 名项目资助研究员） 涉及血管药理学和生物化学的各个领域 系统、肾脏和癌症。 长期目标是提供 长期资助项目以及新项目的 ESI/MS(/MS) 分析 项目。 新仪器将带来重大进步 了解类二十烷酸和谷胱甘肽在健康和健康方面的作用 疾病。