CORE--MASS SPECTROMETRY

核心——质谱

• 批准号: 6500480
• 负责人: MICHAEL BALAZY
• 金额: $ 31.48万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6500480
• 关键词: biomedical facility; gas chromatography mass spectrometry; mass spectrometry

The Core Mass Spectrometry has continued to provide analytical support for the individual program projects for almost 10 years. Mass spectrometry has been successful used in various studies and has proven to be a valuable and adequate technique for the type of studies proposed in this application. Mass spectrometry is an important aspect of the ongoing research and will be utilized by all program project investigators essentially for two general purposes: (a) quantitative analyses of PO450- derived metabolites of arachidonic acid and carbon monoxide, and (b) qualitative (structural) analyses. We have developed in our laboratory quantitative assays based on isotopic dilution GC/MS for CO, 20-HETE and other subterminal HETEs, isomers of epoxyeicosatrienoic acids (EETs) and also for omega-hydroxylated prostaglandins. Because all samples from individual investigators are analyzed by this dedicated core facility with long experience in mass spectrometry, the quantitative data are obtained in a uniform manner, with high quality control and at optimal cost. Samples are analyzed at the same conditions through the study period. Additionally, we have developed a system for sample handling and analysis that utilizes the instrument and staff time efficiently and reduces operational costs while delivering accurate results in a timely manner. Utilization of various aspects of mass spectrometry is an important feature of this program project proposal. This stems from the recognition that mass spectrometric analysis provide adequate sensitivity, specificity, and selectively required for detection and characterization of sub-picomolar quantities of eicosanoids typically found in the biological material. In particular, metabolites or arachidonic acid formed via cytochrome P450 pathway can only e measured accurately and precisely using GC/MS at subpicomolar levels since no other technique of comparable sensitivity and specificity is currently available for their quantitation. A novel aspect of the Core function is the development of an accurate method for quantitative analysis of CO originating from biological material. Using these methodologies, the Core will support Program Project Investigators in their efforts to answer fundamental questions regarding the role of eicosanoids in the mechanisms of hypertension.

近10年来，核心质谱学一直为各个计划项目提供分析支持。质谱学已被成功地用于各种研究，并已被证明是一种有价值的和适当的技术，适用于本申请中提出的研究类型。质谱学是正在进行的研究的一个重要方面，所有计划项目调查人员将主要将其用于两个一般目的：(A)花生四烯酸和一氧化碳PO450衍生代谢物的定量分析，以及(B)定性(结构)分析。我们在实验室建立了基于同位素稀释GC/MS的CO、20-HETE和其他亚端HETE、环氧二十碳三烯酸(EETs)异构体以及omega-羟基前列腺素的定量分析方法。由于来自单个研究人员的所有样品都由这个在质谱学方面拥有长期经验的专用核心设备进行分析，因此可以以统一的方式、高质量的控制和最佳的成本获得定量数据。在整个研究期间，在相同的条件下对样品进行分析。此外，我们还开发了一个样品处理和分析系统，该系统有效地利用了仪器和工作人员的时间，降低了运营成本，同时及时提供了准确的结果。利用质谱学的各个方面是这一方案项目提案的一个重要特点。这源于人们认识到，质谱分析提供了足够的灵敏度、特异度和选择性地检测和表征生物材料中通常存在的亚皮摩尔量的二十烷类化合物。特别是，通过细胞色素P450途径形成的代谢物或花生四烯酸只能在皮下摩尔水平上用GC/MS准确和精确地测量，因为目前还没有其他具有类似灵敏度和特异度的技术可用于定量。核心功能的一个新方面是开发一种准确的定量分析来自生物材料的一氧化碳的方法。使用这些方法，核心将支持计划项目调查人员努力回答有关二十烷类化合物在高血压机制中的作用的基本问题。