INTEGRATED CELLULAR FLUORESCENCE ANALYSIS FACILITY

本提案的目的是获得设备， 集成的细胞荧光分析设备， 南卡罗来纳州大学。 建议的主要组成部分 设施是荧光激光成像板阅读器（FLIPR）和 FACSCalibur流式细胞仪。 该设施是由 一组11个主要用户在三个学院（医学，牙科医学， 和药物科学）和6个部门内的学院 医学（医学，微生物学，药理学，病理学，生理学， 和实验肿瘤学）。 具体目标如下： 1. 为了获得荧光激光成像读板仪（FLIPR）和 用于MUSC细胞荧光的FACSCalibur分析流式细胞仪 分析设施。 2. 利用这一设施提高生产力 NIH资助的研究人员，以提高研究效率， MUSC研究和培训人员的竞争力。 3. 使用这个设施 促进用户之间的跨学科工作。 该设施满足了灵活和高吞吐量的广泛需求 荧光分析支持许多不同学科的研究。 详细的组织计划，实质性的机构承诺， 支持，以及长期的维护计划，以确保持续的 设施的可行性。 集成蜂窝网络的可用性 荧光分析设施将为 细胞荧光检测新用途的开发 方法论。 它将成为跨学科的平台， 调查和培训，并将大大提高 MUSC研究人员的竞争力。

The purpose of this proposal is to obtain equipment to create an integrated Cellular Fluorescence Analysis Facility at the Medical University of South Carolina (MUSC). The key components of the proposed facility are a Fluorometric Laser Imaging Plate Reader (FLIPR) and a FACSCalibur Analytical Flow Cytometer. This facility is requested by a group of 11 major users in three colleges (Medicine, Dental Medicine, and Pharmaceutical Sciences) and 6 departments within the College of Medicine (Medicine, Microbiology, Pharmacology, Pathology, Physiology, and Experimental Oncology). The specific aims are as follows: 1. To obtain a Fluorometric Laser Imaging Plate Reader (FLIPR) and a FACSCalibur Analytical Flow Cytometer for the MUSC Cellular Fluorescence Analysis Facility. 2. To use this facility to enhance the productivity of NIH-funded investigators and to improve the research efficiency and competitiveness of MUSC research and trainees. 3. To use this facility to foster interdisciplinary work among the users. The facility addresses a broad need for flexible and high throughput fluorescence analyses to support research in many different disciplines. A detailed organizational plan, substantial institutional commitment of support, and a long term plan for maintenance ensures the sustained viability of the facility. The availability of the integrated Cellular Fluorescence Analysis Facility will provide unique opportunities for the development of novel new uses for cellular fluorescence detection methodologies. It will serve as a platform for interdisciplinary investigation and training, and will significantly enhance the competitiveness of MUSC investigators.