AUTOMATED DNA SEQUENCER

自动 DNA 测序仪

• 批准号: 2503106
• 负责人: ANNE YODER
• 金额: $ 12.85万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-03 至 1999-04-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503106
• 关键词: DNA; animal genetic material tag; biomedical equipment purchase; human genetic material tag; nucleic acid sequence

Funds are being sought for the purchase of an automated DNA sequencer plus associated small equipment and software necessary to prepare and analyze DNA sequences. The instrument will be placed in a centrally- located Biotechnology facility and will be available to a community of investigators whose research applications include (but are not limited to) molecular evolution and phylogenetics, identification and characterization of new genes, and the study of mutant genes relative to phenotypic expression. Currently, the major and minor users included in this proposal employ manual radioisotopic methods to generate DNA sequence data. In addition to the health risks and exorbitant costs of waste disposal associated with radioactive material, manual sequencing methods are a severe hindrance to the research productivity of each of the investigators. Automated sequencing, however, annuls the health risks, lowers the overall cost of DNA sequencing and increases throughput by an overwhelming percent over manual methods. The proposed automated DNA sequencing machine will be housed in a multiple-user Biotechnology facility in order to facilitate the consistent maintenance, usage, and financing of the equipment. Minor users will be charged a fee for each sequenced template that is appropriate to recover the costs of technician time, consumable materials, and instrument maintenance. The four major users (i.e., the P.I.'s), in collaboration with the Director of the Biotechnology facility, will form a steering committee to determine the fee for service and scheduling of the machine and will have the option of using the machine on the same fee-for service basis as well as being allowed to individually generate sequencing templates and operate the machine. The offices of the Vice President for Research and Associate Dean for Research at the Medical School at Northwestern have an ongoing commitment to the Biotechnology facility with regard to institutional space, facility director and technician salary, and continued maintenance of the proposed equipment.

正在寻求资金购买自动 DNA 测序仪 加上准备和准备所需的相关小型设备和软件 分析 DNA 序列。 该仪器将被放置在中央 位于生物技术设施并将提供给社区 研究应用包括（但不限于 to) 分子进化和系统发育学、鉴定和 新基因的表征以及相关突变基因的研究 到表型表达。 目前，主要用户和次要用户包括 在本提案中，采用手动放射性同位素方法来生成 DNA 序列数据。 除了健康风险和高昂的费用之外 与放射性物质相关的废物处理，手动排序 方法严重阻碍了每个人的研究生产力 调查人员。 然而，自动测序消除了健康 风险，降低 DNA 测序的总体成本并增加 吞吐量比手动方法高出绝大多数。 拟议的 自动 DNA 测序机将安装在多用户 生物技术设施，以促进一致 设备的维护、使用和融资。 未成年用户会 对每个适合的测序模板收取费用 收回技术人员的时间、消耗材料和成本 仪器维护。 四个主要用户（即 P.I.） 与生物技术设施主任合作，将形成 指导委员会确定服务费用和安排 机器，并且可以选择在同一台机器上使用该机器 按服务收费并允许单独生成 对模板进行排序并操作机器。 副办公室 医学部研究主席兼研究副院长 西北大学持续致力于生物技术 设施方面的机构空间、设施主任和 技术员工资，以及拟议设备的持续维护。