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SPECTROSCOPIC SUITE

光谱套件

基本信息

批准号：
2489090
负责人：
MICHAEL CASCIO
金额：
$ 13.87万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-03-23 至 1999-03-22
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2489090
关键词：
biomedical equipment biomedical equipment purchase circular dichroism spectrometry ultraviolet spectrometry

项目摘要

The acquisition of major state of the art instrumentation should ideally impact on a wide range of scientists in various disciplines. The University of Pittsburgh proposes herein to equip a core spectroscopic facility to provide the scientific community with the means to utilize circular dichroism (CD) spectroscopy to further their respective research. These goals will b e realized through the purchase of an AVIV model 62A DS Circular Dichroism spectrometer equipped with state of the art optics, temperature control, syringe pumps, and fluorescence detection. A Macintosh computer interface provides for control of data collection over wavelength, time, or temperature, and allows for facile analyses. A companion high performance AVIV model 14DS UV-Vis Spectrophotometer provides for accurate corresponding absorbance profiles. CD spectroscopy is a highly useful methodology for the determination of protein secondary structure and/or stability. It is non-destructive towards the sample (i.e. samples are fully recovered after spectroscopy) and requires relatively low quantities of purified material (approximately 100 ug). This proposal describes an array of projects which are currently limited by the absence of such an instrument in the Pittsburgh region. Research and educational activities in fields as diverse as molecular pathogenesis of HIV to chemical engineering of enzymes in non-aqueous environments will be significantly impacted by the arrival of a CD spectrometer. The Protein Core facility of the Department of Molecular Genetics and Biochemistry at the University of Pittsburgh School of Medicine will coordinate the purchase, maintenance and use of the instrument. An advisory committee will determine guidelines for the equitable sharing of this resource which will be open to the scientific community for the advancement of their respective research projects and fields.

最先进的仪器的主要状态的收购，对各个学科的科学家产生了广泛的影响。的匹兹堡大学在此提出装备一个核心分光为科学界提供利用圆二色性（CD）光谱，以进一步各自的研究。这些目标将通过购买AVIV 62 A型来实现，B e DS圆二色光谱仪配备了最先进的光学系统，温度控制、注射泵和荧光检测。一 Macintosh计算机界面提供数据收集控制在波长、时间或温度上，并且允许容易的分析。 AVIV 14 DS型高性能紫外-可见分光光度计提供准确的相应吸光度曲线。 CD光谱法是一种非常有用的测定蛋白质二级结构和/或稳定性。它是非破坏性的朝向样品（即样品在光谱分析后完全回收）并且需要相对少量的纯化材料（约100 μ g）。本提案介绍了一系列目前有限的项目，匹兹堡地区缺乏这样的文书。研究在分子发病机制等不同领域的教育活动从HIV到非水环境中酶的化学工程，会受到CD光谱仪的影响。的分子遗传学系蛋白质核心设施，匹兹堡大学医学院的生物化学将协调仪器的购买、维护和使用。一个咨询委员会将确定公平分享这个资源将向科学界开放，推进各自的研究项目和领域。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Asparagine of z8 insert is critical for the affinity, conformation, and acetylcholine receptor-clustering activity of neural agrin.

z8 插入物的天冬酰胺对于神经聚集蛋白的亲和力、构象和乙酰胆碱受体聚集活性至关重要。

DOI：
10.1074/jbc.m110.130625
发表时间：
2010
期刊：
The Journal of biological chemistry
影响因子：
0
作者：
Tseng,Chao-Neng;Zhang,Lili;Wu,Shey-Lin;Wang,Wen-Fu;Wang,Zuo-Zhong;Cascio,Michael
通讯作者：
Cascio,Michael

Calcium plays a critical role in determining the acetylcholine receptor-clustering activities of alternatively spliced isoforms of Agrin.

钙在确定 Agrin 选择性剪接亚型的乙酰胆碱受体簇活性中起着至关重要的作用。