MODELING OF NEUROTRANSMITTER GATED CHANNELS, RECEPTORS, PROTEINS: STRUC & FUNCT

神经递质门控通道、受体、蛋白质的建模：STRUC

• 批准号: 6282512
• 负责人: MICHAEL CASCIO
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282512
• 关键词: Chordata; Invertebrata; Mammalia; aging; animal tissue; biomedical resource; carbohydrates; cognition; computers; endocrine gland /system; enzymes; immunology; inflammation; minerals; nervous system

The overall goal of this project is to examine, at a molecular level, the correlation between structure and function for several neurotransmitter-gated channels, receptors, and related proteins. Currently, the main focus of the laboratory is the examination of the secondary structure, topology, and functional role of domains and specific residues of the homomeric recombinant human $alph$1 glycine receptor overexpressed in a baculovirus expression system. This inhibitory gated anionic channel is a member of the ligand-gated superfamily, which include the homologous nicotinic acetylcholine receptor, the 5-HT3 serotonin receptor, and the GABA receptor, all of which act in rapid mediation of signal transduction at the synapse. These investigations may provide insight into the general conserved mechanism used in channel design. Analogous studies concerning the NMDA type glumatmate receptors and the gonadotropin releasing hormones (GnRH) receptor will also be conducted. These receptors, too, are members of large families, and may their structural featur may be paradigmatic; the NMDA receptor is a member of the excitatory glutamate receptor family which act in synaptic transmission and excitotoxicity, and the GnRH receptor is a member of the large G protein coupled receptors and is a pivotal role in regulating coordination of the neural and endocrine systems. In addition, crystalliszation trails of EP 24.15 a zinc metalloendopeptidase which degrades GnRH, are also underway.

这个项目的总体目标是检查，在一个分子 水平，结构和功能之间的相关性， 神经递质门控通道、受体和相关蛋白质。 目前，实验室的主要工作是检查 结构域的二级结构、拓扑结构和功能作用， 同源重组人甘氨酸的特定残基 受体在杆状病毒表达系统中过表达。 这 抑制性门控阴离子通道是配体门控阴离子通道的成员。 超家族，其中包括同源烟碱乙酰胆碱 受体，5-HT 3血清素受体和GABA受体，所有这些都是 其在突触处的信号转导的快速介导中起作用。 这些调查可以提供洞察一般保守的 渠道设计中使用的机制。 有关《公约》的类似研究 NMDA型谷氨酸受体与促性腺激素释放激素 （GnRH）受体。 这些受体也是 大家庭的成员，他们的结构特征可能是 典型的; NMDA受体是兴奋性 谷氨酸受体家族，其在突触传递中起作用， 兴奋性毒性，GnRH受体是大G 蛋白偶联受体，在调节 协调神经和内分泌系统。 此外，本发明还提供了一种方法， EP 24.15是一种锌金属内肽酶， 促性腺激素释放激素也在进行中。