LEICA TCSNT CONFOCAL MICROSCOPE

徕卡 TCSNT 共焦显微镜

• 批准号: 2486876
• 负责人: KATHY Kay SVOBODA
• 金额: $ 26.93万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2486876
• 关键词: biomedical equipment; biomedical equipment purchase; confocal scanning microscopy; image processing; imaging /visualization /scanning

The purpose of this application is to acquire an updated confocal microscope for the established Boston University School of Medicine Confocal Microscopy Core Facility. The facility currently houses a Leica confocal laser scanning system that was purchased in 1991. Funds are requested to purchase an Leica TCS NT confocal image analysis work station with an inverted microscope. The option of placing the scanner on an upright microscope is also included, but will be funded from institutional support. The software includes the 3D and physiology programs as several researchers plan to obtain time lapse recordings of live tissue stained with vital dyes, calcium or pH indicators. Currently the Anatomy and Neurobiology Department has allocated space for the microscope, and the Dean of Medicine, Graduate Dean, and Departmental Chairs have promised institutional support. The institutional support will include money to purchase an additional upright microscope and other accessories. Extensive data has been collected on the current user group from 1992-1996. The existing confocal system is used over 800 hrs/year with many months topping 100 hrs. Five primary and eight secondary researcher groups have been identified that will need the new equipment for a broad range of projects. The researchers span many age groups, from young investigators to senior scientists. Most participants have current PHS grant support, and are looking forward to enhancing and extending their research with the equipment with this new microscope for the core facility. Our current machine is only 6 years old, however, the computer system is obsolete and simple repairs take extensive time. In addition, it is limited in the types of specimens that can be viewed as it is equiped with only an argon laser and two photomultiplier tubes.

此应用程序的目的是获取更新的共焦 波士顿大学医学院的显微镜 共聚焦显微镜核心设施。该设施目前设有一个徕卡 共焦激光扫描系统，于1991年购买。资金 要求购买Leica TCS NT共焦图像分析工作站 用倒置显微镜将扫描仪放置在 直立显微镜也包括在内，但将由机构资助 支持.该软件包括3D和生理程序， 研究人员计划获得活体组织染色的延时记录， 活性染料钙或pH指示剂目前，解剖学和 神经生物学系为显微镜分配了空间， 医学院院长、研究生院院长和系主任承诺 机构支持。机构支持将包括资金， 购买额外的立式显微镜和其他配件。广泛 收集了1992-1996年期间关于目前用户群体的数据。的 现有的共焦系统使用超过800小时/年， 最高100小时五个主要研究小组和八个次要研究小组 已经确定，将需要新的设备，为广泛的 项目研究人员跨越了许多年龄组，从年轻的研究人员到 资深科学家。大多数参与者目前有PHS赠款支持， 并期待着加强和扩大他们的研究与 为核心设施配备新的显微镜我们目前 机器只有6岁，然而，计算机系统已经过时， 简单的修理要花很长时间。 此外，它还局限于 类型的标本，可以看到，因为它是配备只有氩气 激光器和两个光电倍增管。