Leica SP5 Confocal Microscope

描述（由申请人提供）：本提案的目标是为贝勒牙科学院（BCD）光学显微镜核心设施的Leica TCS SP 5 MP固定载物台（紫外-可见-红外）共聚焦显微镜获得资金。该仪器将用于在受控环境中检查从细胞到整个生物体的样品的基因表达和蛋白质谱。我们有一台10年前的仪器（Leica SP2），由制造商提供支持，但它无法升级，使用寿命有限。此外，共焦技术已经大大改善，因为BCD购买了他们的旧仪器。在过去的20年里，研究一直是BCD的主要重点。部分原因是巴塞尔公约秘书处是一个独立的机构。该学院在地理上与其附属医学院分开（德克萨斯州A & M医学院，学院站，德克萨斯州距离达拉斯约200英里）。BCD拥有一个相对较大的教师和项目群，致力于教学，生物医学教育和研究。有22名全职教师，15名博士后研究员和14名博士。生物医学科学系的学生从事不同的研究项目。该仪器支持的项目将扩大对选定细胞类型如何在正常环境中改变基因表达或蛋白质功能的基本理解。这些项目将由高素质的教师和学生解决发育生物学（Svoboda，Opperman，Ruest，Feng，Qin，D 'Souza），神经生物学（克雷默，本森），免疫学和细胞生物学基础科学问题（Svoboda，Sharma，本森，Opperman，Feng，Qin）。将这种仪器添加到我们的核心设施的更广泛的影响是，研究生，博士后研究员和教师将有机会获得最先进的仪器。它将提供给所有研究学院，并纳入研究生课程，以促进颅面生物学的基础科学研究。这一核心设施的增加也将加强未来与其他达拉斯和健康科学中心教师的合作，并在未来的教师和研究生招聘援助。

DESCRIPTION (provided by applicant): The goal of this proposal is to obtain funds for a Leica TCS SP5 MP Fixed stage (UV- VIS-IR) confocal microscope for the Baylor College of Dentistry (BCD) light microscopy core facility. This instrument will be used to examine the gene expression and protein profiles of samples from cells to whole organisms in a controlled environment. We have a 10 year old instrument (Leica SP2) that is supported by manufacturer, but it cannot be upgraded and it has a limited lifetime. In addition, confocal technology has improved greatly since BCD purchased their older instrument. Research has been a major emphasis at BCD over the past 20 years. In part, this is because BCD is a stand-alone institution. The college is geographically separated from its affiliated medical school (Texas A & M College of Medicine, College Station, TX is about 200 miles from Dallas). BCD maintains a relatively large group of faculty and programs that are devoted teaching, biomedical education and research. There are 22 full time faculty, 15 postdoctoral fellows and 14 Ph.D. students in the Department of Biomedical Sciences working on different research projects. The projects that will be supported by this instrument will expand the basic understanding of how selected cell types change gene expression or protein functions within their normal environment. These projects will address developmental biology (Svoboda, Opperman, Ruest, Feng, Qin, D'Souza), neurobiology (Kramer, Benson), immunology and cell biology basic science questions (Svoboda, Sharma, Benson, Opperman, Feng, Qin) by highly qualified faculty and their students. The broader impact of adding this instrument to our core facilities is that graduate students, postdoctoral fellows, and faculty will have access to a state of the art instrument. It will be available to all research faculties and incorporated into graduate courses to promote basic science research in craniofacial biology. The addition of this core facility will also enhance future collaborations with faculty from other Dallas and health science center faculty and aid in future faculty and graduate student recruitment.